3952-78-1 Usage
Description
Alizarin-3-methyliminodiacetic acid is a colorimetric dye for the detection of fluoride ions. It reacts with fluoride to form a lilac-blue complex which can be quantified colorimetrically at 620 nm to determine fluoride concentration. Alizarin-3-methyliminodiacetic acid has been used to visualize fluoride deposition and bone mineralization during development in medaka larvae. It is also an inhibitor of inducible nitric oxide synthase (iNOS; IC50 = 35 nM).
Chemical Properties
Brown-orange crystals, insoluble in water
Uses
Different sources of media describe the Uses of 3952-78-1 differently. You can refer to the following data:
1. Alizarin Complexone is used for the determination of fluorine and other anions. ATA is also a potent inhibitor of protein-nucleic acid interactions.
2. Alizarin-3-methyliminodiacetic acid has been used:for live staining of mineralized bone matrixto double-label the bone for dynamic bone histomorphometry at the femoral mid-diaphysis in the ratas a label in histomorphometry of the tibial diaphysis to indicate new bone formation in the rat
3. Alizarin is the main ingredient for the manufacture of the madder lake pigments known to painters as Rose madder and Alizarin crimson. A notable use of alizarin in modern times is as a staining agent in biological research because it stains free calcium and certain calcium compounds a red or light purple color. Alizarin Red is used in a biochemical assay to determine, quantitatively by colorimetry, the presence of calcific deposition by cells of an osteogenic lineage.
Definition
ChEBI: A dihydroxyanthraquinone compound in which the hydroxy groups are at C-1 and C-2 and which has a bis[(carboxymethyl)amino]methyl substituent at the 3-position.
General Description
Alizarin-3-methyliminodiacetic acid is used in the colorimetric detection of fluorine. It is a dye employed to stain mineralized bones of the preserved specimen from vertebrates.
Hazard
Anthraquinones are toxic by ingestion: vomiting, diarrhea, kidney and
liver damage, and coma; Deaths have been reported after children
ingested Rhamnus berries; Has antiretroviral activity;An irritant; Harmful by ingestion, inhalation, or skin
absorption; Toxic if swallowed.
Purification Methods
It is purified by suspending it in 0.1M NaOH (1g in 50mL), filtering the solution and extracting alizarin with 5 successive portions of CH2Cl2. Then add HCl dropwise to precipitate the reagent, stirring the solution in an ice bath. Filter the precipitate onto a glass filter, wash it with cold water and dry it in a vacuum desiccator over KOH [Ingman Talanta 20 135 1973, Beilstein 14 IV 931].
Check Digit Verification of cas no
The CAS Registry Mumber 3952-78-1 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 3,9,5 and 2 respectively; the second part has 2 digits, 7 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 3952-78:
(6*3)+(5*9)+(4*5)+(3*2)+(2*7)+(1*8)=111
111 % 10 = 1
So 3952-78-1 is a valid CAS Registry Number.
InChI:InChI=1/C19H15NO8.2H2O/c21-13(22)7-20(8-14(23)24)6-9-5-12-15(19(28)16(9)25)18(27)11-4-2-1-3-10(11)17(12)26;;/h1-5,25,28H,6-8H2,(H,21,22)(H,23,24);2*1H2
