40345-87-7Relevant academic research and scientific papers
Synthetic chemoselective rewiring of cell surfaces: Generation of three-dimensional tissue structures
Dutta, Debjit,Pulsipher, Abigail,Luo, Wei,Yousaf, Muhammad N.
, p. 8704 - 8713 (2011)
Proper cell-cell communication through physical contact is crucial for a range of fundamental biological processes including, cell proliferation, migration, differentiation, and apoptosis and for the correct function of organs and other multicellular tissues. The spatial and temporal arrangements of these cellular interactions in vivo are dynamic and lead to higher-order function that is extremely difficult to recapitulate in vitro. The development of three-dimensional (3D), in vitro model systems to investigate these complex, in vivo interconnectivities would generate novel methods to study the biochemical signaling of these processes, as well as provide platforms for tissue engineering technologies. Herein, we develop and employ a strategy to induce specific and stable cell-cell contacts in 3D through chemoselective cell-surface engineering based on liposome delivery and fusion to display bio-orthogonal functional groups from cell membranes. This strategy uses liposome fusion for the delivery of ketone or oxyamine groups to different populations of cells for subsequent cell assembly via oxime ligation. We demonstrate how this method can be used for several applications including, the delivery of reagents to cells for fluorescent labeling and cell-surface engineering, the formation of small, 3D spheroid cell assemblies, and the generation of large and dense, 3D multilayered tissue-like structures for tissue engineering applications.
Synthesis of sugar oxime ether surfactants
Ewan, Harrison S.,Muli, Christine S.,Touba, Steven,Bellinghiere, Amy T.,Veitschegger, Anne M.,Smith, Travis B.,Pistel, William L.,Jewell, William T.,Rowe, Rebecca K.,Hagen, John P.,Palandoken, Hasan
, p. 4962 - 4965 (2014)
A straightforward synthesis of a novel class of sugar surfactants is described. The key step is the chemoselective condensation of a hydrophobic alkoxyamine with the resident aldehyde/ketone moiety on a hydrophilic sugar. Neither protection/deprotection of the sugars nor extensive product purification is required. The method allows for the facile adjustment of hydrophobic and hydrophilic domains of the sugar oxime ether surfactant and uses inexpensive, readily accessible, and renewable materials.
Method for synthesizing azanol
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Paragraph 0157; 0192; 0198, (2016/10/08)
The invention relates to a hydroxylamine synthesis method. The hydroxylamine synthesis method comprises the following steps: (A) enabling alcohol to react with alkyl sulfonyl halide in the presence of an acid-binding agent to obtain sulphonate; (B) enabling the obtained sulphonate in the step (A) to react with N-hydroxycyclodiimide in the presence of alkali to generate alkylate of the N-hydroxycyclodiimide; and (C) enabling the alkylate obtained in the step (B) to react with an aminolysis reagent or a hydrazinolysis reagent to obtain the hydroxylamine. The method is high in yield and suitable for large-scale industrial hydroxylamine synthesis.
Synthesis of fluorescent molecular probes based on cis-cinnamic acid and molecular imaging of lettuce roots
Fukuda, Hiroshi,Nishikawa, Keisuke,Fukunaga, Yukihiro,Okuda, Katsuhiro,Kodama, Kozue,Matsumoto, Kenji,Kano, Arihiro,Shindo, Mitsuru
supporting information, p. 6492 - 6498 (2016/09/23)
We synthesized azo dye- and fluorescence-labeled cis-cinnamic acid analogues possessing inhibitory activity against lettuce root growth and a trans-isomer without bioactivity as a control probe. The radicles incubated with the azo dye-labeled analogue were stained red, with their tips especially deeply dyed. The fluorescent images of the radicles incubated with each of these molecular probes depicted that the root cap was fluorescence-stained. However, images of the control radicles prepared by staining with the trans-isomer fluorescent probe did not show emission at the root cap. These contrasts suggest specific localization of the cis-cinnamate analogue at the columella cells.
Compositions and methods for promoting liposomal and cellular adhesion
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Page/Page column 16, (2015/07/22)
The present application describes compounds, compositions and methods for incorporating chemoselective and bio-orthogonal complementary functional groups into liposomes. The present application also describes various uses of these modified liposomes inclu
Cell-surface engineering by a conjugation-and-release approach based on the formation and cleavage of oxime linkages upon mild electrochemical oxidation and reduction
Pulsipher, Abigail,Dutta, Debjit,Luo, Wei,Yousaf, Muhammad N.
supporting information, p. 9487 - 9492 (2015/01/06)
We report a strategy to rewire cell surfaces for the dynamic control of ligand composition on cell membranes and the modulation of cell-cell interactions to generate three-dimensional (3D) tissue structures applied to stem-cell differentiation, cell-surfa
Amine exchange in formamidines: An experimental and theoretical study
Capela, Marinha Df.,Mosey, Nicholas J.,Xing, Liyan,Wang, Ruiyao,Petitjean, Anne
supporting information; experimental part, p. 4598 - 4612 (2011/06/24)
N-H-containing formamidines combine a reasonably strong association to carboxylic acids to form complexes of well-defined geometries with a simultaneous proton-induced electrophilicity enhancement that allows for the exchange of their amine portion. The N
