41554-29-4Relevant academic research and scientific papers
New assay of α-l-rhamnosidase
Karni?ová Potocká, Elena,Mastihubová, Mária,?i?ová, Iveta,Mastihuba, Vladimír
, p. 167 - 174 (2018)
Abstract: Free rutinose was prepared by enzymatic hydrolysis of rutin using defatted seed meal from tartary buckwheat. This disaccharide was used as substrate in spectrophotometric assay of α-l-rhamnosidase. The assay is based on hydrolysis of rutinose and subsequent determination of released glucose by a standard glucose oxidase assay kit. The method is easy to perform and requires no expensive equipment. The assay was applied in α-l-rhamnosidase estimation in ten commercial enzyme preparations and compared with standard assay on chromogenic substrate.
