4234-44-0Relevant academic research and scientific papers
Structural elucidation, antioxidant and immunomodulatory activities of a novel heteropolysaccharide from cultured Paecilomyces cicadae (Miquel.) Samson
Wang, Yanbin,He, Pengfei,He, Liang,Huang, Qingrong,Cheng, Junwen,Li, Weiqi,Liu, Yu,Wei, Chaoyang
, p. 270 - 281 (2019/04/17)
The fine structure and chain conformation of a heteropolysaccharide (PCIPS3) from mycelium of Paecilomyces cicadae were investigated via the analysis of HPLC, IR, methylation, NMR spectroscopy and multiangle light scattering. It was determined to be a 2.23 × 104 g/mol heteropolysaccharide primarily composed of glucose, galactose and mannose in a molar ratio of 23.8:2.1:1.0. The PCIPS3 backbone consisted of 1,4-linked α-D-Glcp and 1,4-linked 6-O-Me-α-D-Glcp residues, which were occasionally interrupted by branched β-Galf residues through 1,6-linkage. Moreover, the α (0.60) from Mark–Houwink–Sakurada (MHS) equation suggested that PCIPS3 adopted a flexible chain conformation in 0.1 mol/L NaNO3 at 25 °C. The worm-like chains model parameters for PCIPS3 were estimated as following: ML = 437 nm?1, q = 0.46 nm and 0.79 nm, which were further evidenced by AFM. Furthermore, PCIPS3 showed excellent scavenging capacities of 2,2-diphenyl-1-picrylhydrazyl radical, superoxide radical, hydroxyl radical, ORAC radical and moderate immunomodulatory activity.
Structure and anti-tumor activity of a high-molecular-weight polysaccharide from cultured mycelium of Cordyceps gunnii
Zhu, Zhen-Yuan,Liu, Nian,Si, Chuan-Ling,Liu, Yang,Ding, Li-Na,Jing, Chen,Liu, An-Jun,Zhang, Yong-Min
experimental part, p. 1072 - 1076 (2012/06/16)
Cordyceps gunnii (berk.) Berk (C. Gunnii) is well known as a Chinese rare caterpillar fungus and has similar pharmacological activity with C. sinensis. In this work, a high-molecular-weight polysaccharide (CPS) was isolated and purified from the mycelia of C. gunnii. The total sugar content of CPS was amounted to 92.84%. The result of HPLC indicated that CPS was a homogeneous polysaccharide. The estimated average molecular weight of CPS was 3.72 × 106 Da. The specific rotation of CPS was recorded αD25=+134.2°. Its characteristic was determined by chemical analysis, gas chromatography, IR spectroscopy and NMR data. The results showed that CPS was mainly composed of glucose, and a small amount of rhamnose, arabinose, xylose, mannose and galactose with a molar ratio of Rha:Ara:Xyl:Man:Glu:Gal = 3.0:2.6:1.0:1.3:106.0:2.8. The main chain of CPS was majorly composed of α-(1 → 4) glucose. The tumor inhibition ratio on K562 cell by CPS was 56.65%.
Bioengineering of Leuconostoc mesenteroides glucansucrases that gives selected bond formation for glucan synthesis and/or acceptor-product synthesis
Kang, Hee Kyoung,Kimura, Atsuo,Kim, Doman
experimental part, p. 4148 - 4155 (2011/10/30)
The variations in glucosidic linkage specificity observed in products of different glucansucrases appear to be based on relatively small differences in amino acid sequences in their sugar-binding acceptor subsites. Various amino acid mutations near active sites of DSRBCB4 dextransucrase from Leuconostoc mesenteroides B-1299CB4 were constructed. A triple amino acid mutation (S642N/E643N/V644S) immediately next to the catalytic D641 (putative transition state stabilizing residue) converted DSRBCB4 enzyme from the synthesis of mainly α-(1→6) dextran to the synthesis of α-(1→6) glucan containing branches of α-(1→3) and α-(1→4) glucosidic linkages. The subsequent introduction of mutation V532P/V535I, located next to the catalytic D530 (nucleophile), resulted in the synthesis of an α-glucan containing increased branched α-(1→4) glucosidic linkages (approximately 11%). The results indicate that mutagenesis can guide glucansucrase toward the synthesis of various oligosaccharides or novel polysaccharides with completely altered linkages without compromising high transglycosylation activity and efficiency.
Glucomannans of serissa serissoides stem
Zhao, Hanmin,Zhou, Min,Huang, Meiyan,Wang, Kuiwu,Wei, Wanxing
experimental part, p. 176 - 178 (2011/09/19)
One β-D-glucomannan was isolated from Serissa serissoides (DC.) Druce. The average molecular weight of the polysaccharide was established to be 2.9 × 103 Da. The structure of the polysaccharide from S. serissoides (SSP) was investigated by using sugar and methylation analysis, infrared spectroscopy, mass spectrometry, and NMR spectroscopy. The experiments revealed that SSP consisted of β-D-Glcp and β-D-Manp units in the ratio of approximately 1.1:1 with β-D-(1→4)-Glcp and β-D-(1→2) Manp linkages
Chemical properties and antiulcerogenic activity of a galactomannoglucan from Syagrus oleracea
da Silva, Bernadete Pereira,Parente, José Paz
experimental part, p. 1076 - 1080 (2011/12/03)
A galactomannoglucan (GMG) with an estimated weight-average molar mass of 415,000. g/mol was obtained from an aqueous extract of the mesocarp of fruits of Syagrus oleracea (Mart.) Becc. by fractionation by Sephacryl S-300 HR and Sephadex G-25. Chemical and spectroscopic studies indicated that GMG has a chain of (1 → 4)-linked β-d-mannopyranosyl residues attached to an initial chain of (1 → 3)-linked β-d-galactopyranosyl residues and a terminal chain of (1 → 4)-linked α-d-glucopyranosyl residues which comprised galactose, mannose and glucose in the molar ratio of 30:33:37. Results of the present study indicated that the polysaccharide GMG of S. oleracea significantly inhibited gastric lesions induced by ethanol, showing a gastroprotective property.
Structure characterization of polysaccharide isolated from the fruiting bodies of Tricholoma matsutake
Ding, Xiang,Feng, Su,Cao, Mei,Li, Mao-tao,Tang, Jie,Guo, Chun-xiao,Zhang, Jie,Sun, Qun,Yang, Zhi-rong,Zhao, Jian
experimental part, p. 942 - 947 (2011/12/02)
A novel heteropolysaccharide was isolated from the fruiting bodies of Tricholoma matsutake through DEAE-cellulose column and Sephadex G-100 column. The T. matsutake polysaccharide (TMP-A) had a molecular weight of 8.89 × 104 Da and was mainly composed of 4-D-Glc:4,6-D-Glc:3-D-Gal:T-D-Xyl and with the ratio of 7:1:1:1. The structural features of TMP-A were investigated by a combination of total hydrolysis, methylation analysis, gas chromatography-mass spectrometry (GC-MS), scanning electron microscope (SEM), infrared (IR) spectra, nuclear magnetic resonance (NMR) spectroscopy and dynamical analysis of the atomic force microscope (AFM) studies. The results indicated that TMP-A had a backbone of 1,4-β-glucopyranos which branches at O-6 composed of an (1 → 3)-α-galactopyranose residue and terminated with α-xylopyranose residue. Chain conformation study showed that the polysaccharide took random coil compact conformation.
