42613-33-2

Basic information

• Product Name: DISPASE I
• Synonyms: protease from bacillus polymyxa cell*culture test;Proteinase, Bacillus polymyxa neutral;PROTEASE FROM BACILLUS POLYMYXA CELL*CULTURE TESTED;Dispase from Bacillus polymyxa;Dispase II,Protease from Bacillus polymyxa;Dispase I,Protease from Bacillus polymyxa;Bacillus polymyxa Neutral proteinase;Paenibacillus polymyxa neutral protease
• CAS NO: 42613-33-2
• Molecular Formula: NULL
• Molecular Weight: 0
• EINECS: 255-914-4
• Product Categories: 3.4.x.x Peptidases;3.x.x.x Hydrolases;Application Index;Proteases&Protein Sequencing;ProteasesEnzymes, Inhibitors, and Substrates;Proteolytic Enzymes and Substrates;Proteolytic EnzymesEnzyme Class Index
• Mol File: 42613-33-2.mol
• Article Data: 0

Chemical Properties

• Appearance: /
• Storage Temp.: 2-8°C
• Solubility: 10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble
• CAS DataBase Reference: DISPASE I(CAS DataBase Reference)
• NIST Chemistry Reference: DISPASE I(42613-33-2)
• EPA Substance Registry System: DISPASE I(42613-33-2)

Safety Data

• Hazard Codes: Xn
• Statements: 36/37/38-42
• Safety Statements: 22-26-45-36/37-24
• WGK Germany: 3
• RTECS: UK9540000
• Hazardous Substances Data: 42613-33-2(Hazardous Substances Data)

Usage

Uses

Different sources of media describe the Uses of 42613-33-2 differently. You can refer to the following data:
1. The enzyme from Sigma has been used in developing a protocol for ex vivo culture of mouse embryonic mammary buds. It has been used in the treatment of rat heart pieces during the isolation of mitochondria from rat heart. It has also been used for the isolation of dental pulp stem cells (DPSCs) by enzymatic hydrolysis. These cells have been compared with DPSCs isolated by explant method to analyse their stem cell and differentiation properties.Dispase? II has been used for Fluorescence-Activated Cell Sorting (FACS). It has also been used for separating visceral yolk sac layers.
2. Dispase I has been used in a study to assess the effect of amniotic membrane on wound size in the early stages of the healing process. Dispase I has also been used in a study to investigate a dityrosine-based substrate for a protease assay.

Biochem/physiol Actions

Dispase? II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It may be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes. It can also be used to prevent clumping in suspension cultures. This protease cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin. Dispase? II is specific for the cleavage of Leucine-Phenylalanine bonds. Ca2+, Mg2+, Mn2+, Fe2+, Fe3+ and Al3+ activate the enzyme. EDTA, EGTA, Hg2+ and other heavy metals inhibit the enzyme activity. The enzyme contains 1g-atom of zinc per g-mol of purified enzyme. If this zinc component is removed by chelating agents such as EDTA or EGTA, an inactive apoenzyme is obtained. The enzyme is not inhibited by serum.