42859-94-9Relevant academic research and scientific papers
Combined chemical-enzymatic assembly of aminoglycoside derivatives with N-1-AHB side chain
Nudelman, Igor,Chen, Lilach,Llewellyn, Nicholas M.,Sahraoui, El-Habib,Cherniavsky, Marina,Spencer, Jonathan B.,Baasova, Timor
experimental part, p. 1682 - 1688 (2009/08/12)
A series of unprotected pseudo-disaccharides and pseudo-trisaccharides of 2-deoxystreptamine-containing aminoglycosides have been selectively acylated at the N-1 position with the valuable (S)-4-amino-2-hydroxybutanoyl (AHB) pharmacophore by using the recombinant BtrH and BtrG enzymes from butirosin biosynthesis in combination with a synthetic acyl donor. The process was optimized by performing two enzymatic steps in a sequential manner without purification of the intermediate product.
