4307-76-0Relevant academic research and scientific papers
Determination of enantiomeric purity of commercial 14C- and 3H- labeled L-α-amino acids
LeFevre, Joseph W.,Bonzagni, Neil J.,Chappell, Lara L.,Clement, David J.,Albro, Jeb R.,Lezynski, Denise M.
, p. 477 - 489 (2007/10/03)
The enantiomeric purity of twelve commercial 14C- and 3H-labeled L- α-amino acids was determined using reverse isotope dilution analysis. The technique utilized reversed-phase (RP) thin-layer chromatography (TLC) and beta-cyclodextrin (β-CD) in the mobile phase to separate D- and L-amino acids as their 5-dimethylamino-1-naphthalene sulfonyl (dansyl, DNS) derivatives. In all cases, the L-amino acid was contaminated with the D- isomer. This is the first report of the resolution of N-DNS-DL-tyrosine and N-(α)-DNS-DL-lysine using this methodology.
