4368-01-8Relevant articles and documents
Oxygen-18 kinetic isotope effect studies of the tyrosine hydroxylase reaction: Evidence of rate limiting oxygen activation
Francisco, Wilson A.,Tian, Gaochao,Fitzpatrick, Paul F.,Klinman, Judith P.
, p. 4057 - 4062 (2007/10/03)
Tyrosine hydroxylase converts tyrosine to dihydroxyphenylalanine utilizing a tetrahydropterin cofactor and molecular oxygen. Previous deuterium isotope effect studies have raised the possibility that the activation of oxygen might be the rate-limiting step for this reaction. To test the validity of this proposal, we have measured the 18O kinetic isotope effects for the tyrosine hydroxylase reaction as a function of amino acid substrate, tetrahydropterin derivative, and pH. The measured 18O isotope effects are nearly constant in every condition tested with an average value of 1.0175 ± 0.0019. These results are consistent with a change in the bond order to oxygen in the rate determining step. Furthermore, the isotope effects measured with the coupled substrate 4-methoxyphenylalanine and the completely uncoupled substrate 4-aminophenylalanine are identical, implying the same rate determining step independent of whether oxygen activation is coupled to substrate hydroxylation. The results of these studies provide strong support for a rate limiting reductive activation of molecular oxygen, most likely via a one-electron transfer from the tetrahydropterin to form superoxide anion as the first reactive intermediate.
