439600-29-0Relevant academic research and scientific papers
A New Method for the Preparation of Modified Oligonucleotides
Gorchs, Olga,Hernandez, Marta,Garriga, Lourdes,Pedroso, Enrique,Grandas, Anna,Farras, Jaume
, p. 1827 - 1830 (2002)
(Equation Presented) N-Nitrothymidine can be transformed into a phosphoramidite building block suitable for oligonucleotide synthesis using the standard phosphite triester solid-phase approach. The N-nitrothymidine residues remain stable during the elongation cycles and react smoothly with primary amines, furnishing oligonucleotides containing N3-modified thymidines. A number of N3-substituted oligonucleotides have been prepared using this methodology, some of them incorporating aminoalkyl or hydroxyalkyl groups.
