4467-68-9 Usage
Uses
Used in Pharmaceutical Research:
H-LEU-4M-BETANA HCL is used as a research tool for the study of protease activities within cells. It aids in understanding the role of aminopeptidase M and leucine aminopeptidase in various biological processes and their potential as therapeutic targets.
Used in Drug Development:
H-LEU-4M-BETANA HCL serves as a valuable compound in the development of drugs targeting proteases, particularly aminopeptidase M and leucine aminopeptidase. By providing insights into the substrate specificity and enzymatic activity of these proteases, it can contribute to the design of more effective and selective inhibitors or activators.
Used in Diagnostic Applications:
In the field of diagnostics, H-LEU-4M-BETANA HCL can be employed as a diagnostic marker for the assessment of protease activity levels in various diseases. This can help in the early detection and monitoring of conditions associated with abnormal protease activity, such as certain cancers and inflammatory diseases.
Used in Biochemical Assays:
H-LEU-4M-BETANA HCL is used as a substrate in biochemical assays to measure the activity of aminopeptidase M and leucine aminopeptidase. These assays are crucial for determining the enzymatic properties of these proteases and their potential involvement in disease processes.
Used in Cell Biology:
In cell biology, H-LEU-4M-BETANA HCL is used as a cell-permeable substrate to study the intracellular localization and function of aminopeptidase M and leucine aminopeptidase. This can provide insights into the cellular mechanisms regulated by these proteases and their role in maintaining cellular homeostasis.
Used in Enzymology:
H-LEU-4M-BETANA HCL is used as a substrate in enzymology to investigate the catalytic properties of aminopeptidase M and leucine aminopeptidase. This can help in understanding the molecular mechanisms underlying their function and in the development of novel inhibitors or activators with potential therapeutic applications.
in vitro
l-leucine 4-methoxy-β-naphthylamide was identified as a cell-permeable substrate for aminopeptidase m and leucine aminopeptidase that was developed for intracellular analysis of protease activities [1]. in a previous method-developing study, peptide derivatives of 4-methoxy-β-naphthylamine including l-leucine 4-methoxy-β-naphthylamide were incubated in microtiter plates together with nitrosalicylaldehyde. selectivity was achieved by running parallel assays containing inhibitors partially selective for each of peptide derivatives. this method was successfully validated by measurements in cells isolated from cathepsin b-/--, k-/--, and l-/-- mice [2].
references
[1] monis, b. ,wasserkrug, h. and seligman, a.m. comparison of fixatives and substrates for aminopeptidase. journal of histochemistry and cytochemistry 13(6), 503-509 (1965).[2] rüttger, a. ,mollenhauer, j.,lser, r., et al. microplate assay for quantitative determination of cathepsin activities in viable cells using derivatives of 4-methoxy-β-naphthylamide. biotechniques 41(4), 469-473 (2006).
Check Digit Verification of cas no
The CAS Registry Mumber 4467-68-9 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 4,4,6 and 7 respectively; the second part has 2 digits, 6 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 4467-68:
(6*4)+(5*4)+(4*6)+(3*7)+(2*6)+(1*8)=109
109 % 10 = 9
So 4467-68-9 is a valid CAS Registry Number.
InChI:InChI=1/C17H22N2O2.ClH/c1-11(2)8-15(18)17(20)19-13-9-12-6-4-5-7-14(12)16(10-13)21-3;/h4-7,9-11,15H,8,18H2,1-3H3,(H,19,20);1H
