4484-59-7Relevant academic research and scientific papers
Liposome Enhanced Detection of Amyloid Protein Aggregates
Kocsis, Istvan,Sanna, Elena,Hunter, Christopher A.
, p. 647 - 650 (2021/02/06)
Thioflavin-T is used to image amyloid aggregates because of the excellent turn-on fluorescence properties, but binding affinities are low. By mounting multiple dye units on the surface of a vesicle, the binding affinity for α-synuclein fibrils is increased by three orders of magnitude, and the optical response is increased. Cooperative interactions of the dye headgroup and lipid with the protein provide a general strategy for the construction of multivalent amyloid probes based on vesicles.
Combinatorial synthesis of PEG oligomer libraries
-
Page/Page column 9, (2010/02/15)
A simple chain-extending approach was established for the scale-up of the monoprotected monodisperse PEG diol materials. Reactions of THP-(OCH2CH2)n—OMs (n=4, 8, 12) with a large excess of commercially available H—(OCH2CH2)n—OH (n=1-4) under basic conditions led to THP-(OCH2CH2)n—OH (n=5-15). Similarly, Me-(OCH2CH2)n—OH (n=4-11, 13) were prepared from Me-(OCH2CH2)n—OMs (n=3, 7, 11). For the chain elongation steps, 40-80% yields were achieved through extraction purification. PEG oligomer libraries I and II were generated in 50-95% overall yields by alkylation or acylation of THP-(OCH2CH2)n—OH (n=1-15) followed by deprotection. Alkylation of Me-(OCH2CH2)n—OH (n=1-11, 13) with X—(CH2)m—CO2R (X=Br or OMs) and subsequent hydrolysis led to PEG oligomer library III in 30-60% overall yields. Combinatorial purification techniques were adapted to the larger-scale library synthesis. A total of 498 compounds, each with a weight of 2-5 g and a minimum purity of 90%, were synthesized.
Mutarotation of Glucose Derivatives in Solutions of Surfactans in Organic Solvents: Co-operativity and Bimodal Catalytic Behaviour
Bethell, Donald,Galsworthy, Peter J.,Jones, Keith
, p. 2035 - 2044 (2007/10/02)
The mutarotation of glucose, 2,3,4,6-tetra-O-methylglucose, 3-O-hexyl-, 3-O-dodecyl-, 4,6-O-butylidene, 4,6-O-hexylidene, and 4,6-O-decylidene-glucose has been studied kinetically in aqueous solution and in the following surfactant-solvent systems: AOT-heptane, AOT-CHCl3, CPC-CHCl3, CTAC-CHCl3, CPS-CHCl3 and C16E6-tetradecane.Below a low critical surfactant concentration, mutarotation is undetectably slow, but above it the rate increases, usually in a sigmoidal fashion reaching a maximum value at concentrations above ca. 40 mmol l-1.Maximum rates are usually less than those observed in water except for AOT-containing systems which often, but not always, give higher rates.The dependence of rate on surfactant concentration does not in general fit the pseudophase model of micellar catalysis, but can be treated using Piszkiewicz's co-operativity model.This indicates in a number of cases bimodal catalytic behaviour, a non-co-operative mode at concentrations just above the critical level, and a co-operative mode giving more efficient catalysis at higher concentrations.For AOT-heptane the bimodal pattern is reversed and evidence is presented that the co-operative effects observed at low surfactant concentrations propbably represent catalysis in premicellar aggregates.N.m.r. spectroscopic stiudies (δ, T1) of the protons of water solubilised in the surfactant-solvent systems are reported but do not show helpful correlations with catalytic efficiency in the system studied.A better guide to catalytic efficiency is provided by solvatochromic measurements using N-hexadecylpyridinium iodides incorporated into the catalytic aggregates to report on the polarity in the interior.A possible extension to this approach is discussed.
