45214-88-8

Basic information

• Product Name: H-Met-Lys-OH
• Synonyms: H-Met-Lys-OH
• CAS NO: 45214-88-8
• Molecular Formula: C11H23N3O3S
• Molecular Weight: 277.38362
• Mol File: 45214-88-8.mol
• Article Data: 1

Chemical Properties

• Appearance: /
• CAS DataBase Reference: H-Met-Lys-OH(CAS DataBase Reference)
• NIST Chemistry Reference: H-Met-Lys-OH(45214-88-8)
• EPA Substance Registry System: H-Met-Lys-OH(45214-88-8)

Safety Data

• Hazardous Substances Data: 45214-88-8(Hazardous Substances Data)

Upstream product

• 721928-37-6 7-(N-formylmethionyl-L-lysyl)amino-4-methylcoumarin 

Relevant articles and documents

Slow-binding inhibition of peptide deformylase by cyclic peptidomimetics as revealed by a new spectrophotometric assay

A new spectrophotometric/fluorimetric assay for peptide deformylase (PDF) has been developed by coupling the PDF reaction with that of dipeptidyl peptidase I (DPPI) and using N-formyl-Met-Lys-AMC as substrate. Removal of the N-terminal formyl group by PDF renders the dipeptide an efficient substrate of DPPI, which subsequently removes the dipeptidyl units to release 7-amino-4-methylcoumarin as the chromophore/fluorophore. The PDF reaction is conveniently monitored on a UV-Vis spectrophotometer or a fluorimeter in a continuous fashion. The utility of the assay was demonstrated by determining the catalytic activity of PDF and the inhibition constants of PDF inhibitors. These studies revealed the slow-binding behavior of a previously reported macrocyclic PDF inhibitor. This method offers several advantages over the existing PDF assays and should be particularly useful for screening PDF inhibitors in the continuous fashion.