4527-92-8

Usage

Uses

Used in Biological Studies:
Adenosine 2',3'-cyclic phosphate 5'-phosphate is used as a sulfuryl group acceptor in a reaction catalyzed by phenol sulfotransferase. This reaction is essential for the biosynthesis of various biologically active compounds, including neurotransmitters and hormones.
Used in Cellular Function Regulation:
Adenosine 2',3'-cyclic phosphate 5'-phosphate is used in biological studies to investigate the effect of adenine nucleotides on myo-inositol-1,4,5-trisphosphate-induced calcium release. This process is crucial for the regulation of intracellular calcium levels, which in turn affects various cellular functions, such as muscle contraction, neurotransmission, and gene expression.

Upstream product

• 128-09-6 N-chloro-succinimide 
• 42824-16-8 pyridine-SO₃ complex 
• 61-19-8 5'-adenosine monophosphate 

Relevant academic research and scientific papers

Phosphorylation of Nucleotides with Inorganic Cyclo-Triphosphate

Phosphorylation of nucleotides (nucleoside 3'- and 5'-monophosphates, and 2'-deoxynucleoside 5'-monophosphates) with inorganic sodium cyclotriphosphate (P3m) was studied in aqueous solutions under various conditions (mixing ratio of P3m to nucleotides, pH, reaction temperature, and time). (1) Unprotected nucleoside 5'-monophosphates (5'-NMP's) were easily phosphorylated at the cis-2',3'-diol by P3m to form selectively nucleoside 2',5'-bis(monophosphate) (2',5'-NDP's), nucleoside 3',5'-bis(monophosphate) (3',5'-NDP's), and nucleoside 2',3'-cyclic 5'-bis(monophosphate) (cNDP's). (2) The phosphorylation of 5'-NMP's was strongly dependent on mixing ratio, pH, reaction temperature, and time.Under conditions of high mixing ratios of P3m to 5'-NMP's (5:1 - 10:1), high pH (12), and room temperature, 92 -98percent of 5'-NMp's was converted into 3',5'-NDP's and 2',5'-NDP's in roughly equimolar quantities. (3) Small quantities (5 -8percent) of cNdP's were formed at the initial stage of reaction of 5'-NMP's with P3m but in the course of the reaction for a long period, cNDP's were hydrolyzed to 2',5'-NDP's and 3',5'-NDP's. (4) Nucleoside 3'-monophosphates (3'-NMP's) and 2'-deoxynucleoside 5'-monophosphates (dNMP's) could not be phosphorylated by P3m, which indicates that the presence of hydroxyl groups at both 2'- and 3'-positions on nucleotides is indispensable for the phosphorylation of nucleotides with P3m. (5) The mechanism of the formation of 2',5'-NDP's, 3',5'-NDP's, and cNDP's in the phosphorylation of 5'-NMP's with P3m is discussed.