L-Leucine-p-nitroanilide is a synthetic compound that serves as a substrate for certain enzymes, particularly those involved in the hydrolysis of peptide bonds. It is composed of L-leucine, an essential amino acid, and p-nitroaniline, a derivative of aniline with a nitro group attached to the para position. L-Leucine-p-nitroanilide is often used in biochemical research to study the activity and specificity of proteolytic enzymes, such as trypsin and chymotrypsin. When these enzymes cleave the peptide bond in L-Leucine-p-nitroanilide, they release the p-nitroaniline moiety, which can be easily detected and quantified due to its distinct color and absorbance properties. This allows researchers to assess enzyme kinetics, inhibitor effects, and other aspects of enzyme function in a controlled laboratory setting.
The CAS Registry Mumber 4578-93-2 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 4,5,7 and 8 respectively; the second part has 2 digits, 9 and 3 respectively. Calculate Digit Verification of CAS Registry Number 4578-93: (6*4)+(5*5)+(4*7)+(3*8)+(2*9)+(1*3)=122 122 % 10 = 2 So 4578-93-2 is a valid CAS Registry Number.
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