461426-36-8Relevant articles and documents
Syntheses of labeled vitamers of folic acid to be used as internal standards in stable isotope dilution assays
Freisleben, Achim,Schieberle, Peter,Rychlik, Michael
, p. 4760 - 4768 (2007/10/03)
[2H4]Folic acid was synthesized by deuterating p-aminobenzoic acid, which was then coupled to glutamic acid and 6-formylpterin, Using [2H4]folic acid as starting component enabled the preparation of labeled vitamers tetrahydrofolate, 5-formyltetrahydrofolate, 5-methyltetrahydrofolate, and 10-formylfolate which were characterized by electrospray mass spectrometry and collision-induced dissociation. The mass spectrometric studies confirmed that the compounds could be used as internal standards in stable isotope dilution assays.