464190-00-9Relevant academic research and scientific papers
Tight binding ligand approach to oligosaccharide-grafted protein
Totani, Kiichiro,Matsuo, Ichiro,Ito, Yukishige
, p. 2285 - 2289 (2004)
A novel type of artificial glycoprotein was developed, by using dihydrofolate reductase (DHFR) and methotrexate (MTX) as a protein-ligand pair. Various oligosaccharides linked to MTX were shown to bind tightly with DHFR and afforded oligosaccharide-grafted protein, which could be isolated easily by lectin beads.
Synthesis of an octamannosyled glycan chain, the key oligosaccharide structure in ER-associated degradation
Matsuo, Ichiro,Ito, Yukishige
, p. 2163 - 2168 (2007/10/03)
The high-mannose type decasaccharide (Man8GlcNAc2), the proposed ligand of ER residing mannosidase-like proteins (MLP), and its monoglycosylated homologue (α-Glc1Man8GlcNAc 2) were synthesized. The oligosaccharide assembly was performed in a convergent and stereoselective manner, using three oligosaccharide components, a core trisaccharide having a β-mannoside bond, a liner mannotriose, and a branched mannotetraose.
Desilylation under high pressure
Matsuo, Ichiro,Wada, Megumi,Ito, Yukishige
, p. 3273 - 3275 (2007/10/03)
Cleavage of tert-butyldiphenylsilyl (TBDPS) ether was examined under high pressure conditions (1.0 GPa) using HF/pyridine in DMF. This method proved to be exceptionally suitable for deprotecting the TBDPS group that resides at the sterically hindered posi
