469860-87-5Relevant academic research and scientific papers
Enzymatic syntheses and selective hydrolysis of O-β-d- galactopyranosides using a marine mollusc β-galactosidase
Giordano, Assunta,Tramice, Annabella,Andreotti, Giuseppina,Mollo, Ernesto,Trincone, Antonio
, p. 139 - 143 (2007/10/03)
The use of crude extract of the hepatopancreas of Aplysia fasciata, a large mollusc belonging to the order Anaspidea containing a β-galactosidase activity, was reported for the synthesis of different galactosides. Good yields with polar acceptors and the
Regiospecific synthesis of lactose analog Gal-(β 1,4)-Xyl by transgalactosylation
Fessner, Wolf-Dieter,Juarez Ruiz, Juan Manuel
, p. 739 - 742 (2007/10/03)
A short enzymatic synthesis of disaccharide 4-O-β-D-galactopyranosyl-D-xylose (1) has been developed, which is of interest as a lactose analog for a non-invasive medicinal determination of lactose intolerance. The starting material, benzyl α-D-xyloside, was obtained by a Fischer-type glycosidation of D-xylose with benzyl alcohol, followed by anomeric differentiation of mixed glycosides using a glycosidase from Aspergillus oryzae. From several commercial β-galactosidases, which were screened for their transgalactosylation capacity, the enzyme from Escherichia coli was found to catalyze a virtually regio- and stereospecific galactosyl transfer from donor compounds o-nitrophenyl β-D-galactoside or lactose to the α-D-xyloside. Subsequent hydrogenolytic deprotection furnished desired disaccharide 1.
