Z-Met-O-o-nitrophenylester, also known as Z-Met-ONp, is a synthetic chemical compound commonly used as a substrate in enzymatic assays to study the activity of proteases, particularly cysteine proteases. The compound consists of a methionine residue (Met), a benzyloxycarbonyl (Z) protecting group, and an o-nitrophenyl ester (ONp) moiety. When a protease cleaves the peptide bond between the methionine and the Z group, the o-nitrophenyl ester is released, and its absorbance at 405 nm can be measured, allowing for the quantification of protease activity. Z-Met-ONp is widely used in research and diagnostic applications due to its stability, ease of synthesis, and sensitivity in detecting protease activity.
The CAS Registry Mumber 49689-72-7 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 4,9,6,8 and 9 respectively; the second part has 2 digits, 7 and 2 respectively. Calculate Digit Verification of CAS Registry Number 49689-72: (7*4)+(6*9)+(5*6)+(4*8)+(3*9)+(2*7)+(1*2)=187 187 % 10 = 7 So 49689-72-7 is a valid CAS Registry Number.