502687-91-4Relevant academic research and scientific papers
Synthesis of novel hapten derivatives of 1α,25-dihydroxyvitamin D3 and its 20-epi analogue
Blaehr, Lars K. A.,Bjoerkling, Fredrik,Calverley, Martin J.,Binderup, Ernst,Begtrup, Mikael
, p. 1367 - 1375 (2007/10/03)
Hapten derivatives of 1α,25-dihydroxyvitamin D3 and its 20-epimer were synthesized and conjugated to a carrier protein for raising polyclonal antibodies. The haptens were linked through spacers at C-16, thereby exposing both the A-ring and the side chain of the molecules, to maximize antibody specificity. The spacers were introduced via stereoselective hydroboration of 16-ene intermediates as the key step. In immunoassays, the antibodies raised toward the natural hormone were selective to this compound over derivatives with modifications in the A-ring or the side chain. The antibodies toward the 20-epimer, however, were unable to recognize modifications in the side chain.
