51306-35-5

Basic information

• Product Name: 5-(4,6-DICHLORO-S-TRIAZIN-2-YLAMINO)FLUORESCEIN-HYDROCHLORIDE
• Synonyms: 5-(4,6-DICHLORO-S-TRIAZIN-2-YLAMINO)FLUORESCEIN-HYDROCHLORIDE;5-(4,6-DICHLOROTRIAZINYL)AMINOFLUORESCEIN;5-DTAF;DTAF 2HCL;DTAF HCL;5-((4,6-dichloro-1,3,5-triazin-2-yl)amino)fluorescein;5-(4,6-dichloro-S-triazin-2-ylamino) fluorescein-hcl;4-(4,6-dichloro-s-triazin-2-ylamino)fluorescein 2HCl
• CAS NO: 51306-35-5
• Molecular Formula: C₂₃H₁₂Cl₂N₄O₅
• Molecular Weight: 568.19
• Mol File: 51306-35-5.mol
• Article Data: 8

Chemical Properties

• Melting Point: >325°C
• Boiling Point: 834.4 °C at 760 mmHg
• Flash Point: 458.4 °C
• Appearance: /
• Density: 1.81g/cm³
• Vapor Pressure: 4.24E-29mmHg at 25°C
• Storage Temp.: 2-8°C
• PKA: 9.33±0.20(Predicted)
• CAS DataBase Reference: 5-(4,6-DICHLORO-S-TRIAZIN-2-YLAMINO)FLUORESCEIN-HYDROCHLORIDE(CAS DataBase Reference)
• NIST Chemistry Reference: 5-(4,6-DICHLORO-S-TRIAZIN-2-YLAMINO)FLUORESCEIN-HYDROCHLORIDE(51306-35-5)
• EPA Substance Registry System: 5-(4,6-DICHLORO-S-TRIAZIN-2-YLAMINO)FLUORESCEIN-HYDROCHLORIDE(51306-35-5)

Safety Data

• WGK Germany: 3
• F: 10-21
• Hazardous Substances Data: 51306-35-5(Hazardous Substances Data)

Usage

Uses

Fluorescent reagent for conjugation of fluorescein to proteins, carbohydrates and selected polymers.

InChI:InChI=1/C23H12Cl2N4O5.ClH/c24-20-27-21(25)29-22(28-20)26-10-1-4-14-13(7-10)19(32)34-23(14)15-5-2-11(30)8-17(15)33-18-9-12(31)3-6-16(18)23;/h1-9,30-31H,(H,26,27,28,29);1H

Relevant articles and documents

Single label comparative hybridization

The present invention provides methods of detecting and mapping chromosomal or genetic abnormalities associated with various diseases or with predisposition to various diseases, or to detecting the phenomena of large scale copy number variants. In particular, the present invention provides advanced methods of performing array-based comparative hybridization that allow reproducibility between samples and enhanced sensitivity by using the same detectable label for both test sample and reference sample nucleic acids. Invention methods are useful for the detection or diagnosis of particular disease conditions such as cancer, and detecting predisposition to cancer based on detection of chromosomal or genetic abnormalities and gene expression level. Invention methods are also useful for the detection or diagnosis of hereditary genetic disorders or predisposition thereto, especially in prenatal samples. Moreover, invention methods are also useful for the detection or diagnosis of de novo genetic aberrations associated with post-natal developmental abnormalities.

Assay for mycobacterium avium/intracellulare nucleic acid

Disclosed is a method for determining the presence of Mycobacterium avium complex nucleic acids in a biological sample. In particular, the mig gene of M. avium and the DT1 gene of M. intracellulare are detected, preferably following amplification. In addition, the method distinguishes between species of M. avium and M. intracellulare. Also described are oligonucleotides that can be used as primers to amplify target genes such as mig and DT1 genes and as probes as well as kits containing the oligonucleotides.

Activatable probes and methods for in vivo gene detection

Probes for detecting a target polynucleotide are provided. One aspect provides a molecular beacon probe set wherein the donor molecular beacon comprises a quantum dot and an acceptor molecular beacon comprises at least one reporter. The probes optionally comprise a protein transduction domain, targeting signal, or a combination thereof. Methods for detecting target polynucleotides using the disclosed probes are also provided.