51350-01-7Relevant academic research and scientific papers
Fluorescent probes for use in protein kinase inhibitor binding assay
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Page/Page column 19-21, (2008/06/13)
The invention provides methods relating to a novel screening assay format that can be applied to broad members of the protein kinase gene family. The assay uses a series of labeled, active site probes described herein that can be displaced by an inhibitor agent. The Kd for the inhibitor compound is derived based on the Kd of the probe for the kinase and the dose response of the inhibitor agent. The invention also provides active site probes suitable for use with the screening method.
