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Benzenamine,3,3'-[1,4-butanediylbis(oxy)]bis-, commonly known as 3,3'-Diaminobenzidine (DAB), is a chemical compound that is widely used in immunohistochemistry and molecular biology for the detection of proteins and nucleic acids. It is a white to off-white crystalline powder that is soluble in water and ethanol. DAB functions as a chromogen in various staining techniques, particularly in immunohistochemistry, to visualize the presence of specific antigens. Additionally, it can be used as a substrate for detecting hydrogen peroxide in biological samples. However, due to its potential carcinogenic properties, DAB should be handled with care and proper safety protocols should be followed when using it in laboratory settings.

5226-81-3

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5226-81-3 Usage

Uses

Used in Immunohistochemistry:
Benzenamine,3,3'-[1,4-butanediylbis(oxy)]bisis used as a chromogen in immunohistochemistry for the visualization of specific antigens. It provides a colorimetric reaction that allows for the detection and localization of target proteins within tissue sections, aiding in the diagnosis and study of various diseases and conditions.
Used in Molecular Biology:
In molecular biology, Benzenamine,3,3'-[1,4-butanediylbis(oxy)]bisis used as a substrate for the detection of hydrogen peroxide in biological samples. This application is valuable in research and diagnostic settings, as hydrogen peroxide is an important reactive oxygen species involved in various cellular processes and can be an indicator of oxidative stress or other biological activities.
Used in Research Laboratories:
Benzenamine,3,3'-[1,4-butanediylbis(oxy)]bisis used in research laboratories for the development and optimization of staining techniques and detection methods. Its role as a chromogen and substrate makes it a valuable tool for advancing scientific knowledge and improving diagnostic capabilities.

Check Digit Verification of cas no

The CAS Registry Mumber 5226-81-3 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 5,2,2 and 6 respectively; the second part has 2 digits, 8 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 5226-81:
(6*5)+(5*2)+(4*2)+(3*6)+(2*8)+(1*1)=83
83 % 10 = 3
So 5226-81-3 is a valid CAS Registry Number.

5226-81-3Downstream Products

5226-81-3Relevant academic research and scientific papers

Homo- and hetero-bivalent edrophonium-like ammonium salts as highly potent, dual binding site AChE inhibitors

Leonetti, Francesco,Catto, Marco,Nicolotti, Orazio,Pisani, Leonardo,Cappa, Anna,Stefanachi, Angela,Carotti, Angelo

, p. 7450 - 7456 (2008)

A number of mono- and bis-quaternary ammonium salts, containing edrophonium-like and coumarin moieties tethered by an appropriate linker, proved to be highly potent and selective dual binding site acetylcholinesterase inhibitors with good selectivity over

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