52811-47-9Relevant academic research and scientific papers
Characterization of thermophilic archaeal isopentenyl phosphate kinases
Chen, Mo,Poulter, C. Dale
experimental part, p. 207 - 217 (2010/10/21)
Archaea synthesize isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), the essential building blocks of isoprenoid compounds, from mevalonate (MVA). However, an analysis of the genomes of several members of the Archaea failed to identify genes for the enzymes required to convert phosphomevalonate (PM) to IPP in eukaryotes. The recent discovery of an isopentenyl kinase (IPK) in Methanocaldococcus jannaschii (MJ) suggests a new variation of the MVA pathway where PM is decarboxylated to give isopentenyl phosphate (IP), which is phosphorylated to produce IPP. A blast search using the MJ protein as a probe revealed a subfamily of amino acid kinases that include the fosfomycin resistance protein fomA, which deactivates the antibiotic by phosphorylation of its phosphonate residue in a reaction similar to the conversion of IP to IPP. IPK genes were cloned from two organisms identified in the search, Methanothermobacter thermautotrophicus (MTH) and Thermoplasma acidophilum (THA), and the His-tagged recombinant proteins were purified by Ni-NTA chromatography. The enzymes catalyze the reversible phosphorylation of IP by ATP, Keq=6.3 ± 1. The catalytic efficiencies (V/K) of the proteins were ～2 × 106M-1 s-1. In the reverse direction, ADP was a substrate inhibitor for THAIPK, Ki ADP=58 ±6 μM, but not forMTHIPK. Both enzymes were active over a broad range of pH and temperature. Five compounds, dimethylallyl phosphate, isopentenyl thiolophosphate, 1-butyl phosphate, 3-buten-1-yl phosphate, and geranyl phosphate, were evaluated as alternative substrates for the MTH and THA IP kinases. All of the compounds were phosphorylated, although the catalytic efficiency was low for geranyl phosphate. 2009 American Chemical Society.
Surface properties of butanol phosphate esters in alkali solutions
Du, Zong-Liang,Zhou, Dong-Liang,Chen, Yong,Chen, Min,Zhu, Pu-Xin
experimental part, p. 201 - 206 (2011/10/11)
Phosphate esters of two butanol isomers were synthesized by esterification of n-butanol and isobutanol with phosphorus pentoxide. The surface activity and penetrability of the esters were analyzed by way of surface chemistry and canvas disc wetting tests in different alkaline solutions. The surface tension of the butanol phosphate esters and their sodium salts was found to decrease with increasing alkaline concentration up to 250 g/L NaOH. Furthermore, the products exhibited a good surface activity with proper penetrability even in highly alkaline solutions. The stable surface activity of the esters in concentrated alkali lye interpreted by the adsorption of the molecule from solution owing to the common ion effect. AOCS 2009.
