53832-94-3Relevant academic research and scientific papers
Interference of carbidopa and other catechols with reactions catalyzed by peroxidases
G?sowska-Bajger, Beata,Nishigaya, Yuki,Hirsz-Wiktorzak, Krystyna,Rybczyńska, Anna,Yamazaki, Toshimasa,Wojtasek, Hubert
, p. 1626 - 1634 (2018/04/26)
Background: A number of compounds, including ascorbic acid, catecholamines, flavonoids, p-diphenols and hydrazine derivatives have been reported to interfere with peroxidase-based medical diagnostic tests (Trinder reaction) but the mechanisms of these effects have not been fully elucidated. Methods: Reactions of bovine myeloperoxidase with o-dianisidine, bovine lactoperoxidase with ABTS and horseradish peroxidase with 4-aminoantipyrine/phenol in the presence of carbidopa, an anti-Parkinsonian drug, and other catechols, including L-dopa, were monitored spectrophotometrically and by measuring hydrogen peroxide consumption. Results: Chromophore formation in all three enzyme/substrate systems was blocked in the presence of carbidopa and other catechols. However, the rates of hydrogen peroxide consumption were not much affected. Irreversible enzyme inhibition was also insignificant. Conclusions: Tested compounds reduced the oxidation products or intermediates of model substrates thus preventing chromophore formation. This interference may affect interpretation of results of diagnostic tests in samples from patients with Parkinson's disease treated with carbidopa and L-dopa. General significance: This mechanism allows prediction of interference in peroxidase-based diagnostic tests for other compounds, including drugs and natural products.
Oxidation of carbidopa by tyrosinase and its effect on murine melanoma
Gasowska-Bajger, Beata,Frackowiak-Wojtasek, Bozena,Koj, Sabina,Cichon, Tomasz,Smolarczyk, Ryszard,Szala, Stanislaw,Wojtasek, Hubert
body text, p. 3507 - 3510 (2010/08/06)
Oxidation of the anti-Parkinsonian agent carbidopa by tyrosinase was investigated. The products of this reaction were identified as 3-(3,4-dihydroxyphenyl)-2-methylpropanoic acid and 6,7-dihydroxy-3-methylcinnoline. These results demonstrate that after ox
Further studies on the metabolism of carbidopa, (-) L α hydrazino 3,4 dihydroxy α methylbenzenepropanoic acid monohydrate, in the human, rhesus monkey, dog, and rat
Vickers,Stuart,Hucker,VandenHeuvel
, p. 134 - 138 (2007/10/12)
Major urinary metabolites of carbidopa were identified. Estimates were made based on the recovery of radioactivity or by GLC analysis of pooled urine of the amounts of the urinary metabolites II (2 methyl 3' methoxy 4' hydroxy phenyl propionic acid), III
