53864-51-0Relevant articles and documents
Method for preparing deuterated internal standard substances of tetracyclines and epimers thereof
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Paragraph 0031-0033, (2019/04/17)
The invention discloses a method for preparing deuterated internal standard substances of tetracyclines and epimers thereof. According to the method, a demethylated primary amine intermediate is obtained through three-step reaction including cyclization, ring-opening reaction and reduction reaction, and deuterated paraformaldehyde is subjected to reductive amination to obtain a deuterated internalstandard reagent of tetracyclines finally. Compared with a traditional method for preparing stable isotope labeled tetracycline internal standard reagents through fermentation or total synthesis, themethod has advantages of short steps, simplicity and convenience in operation, high yield, low cost, high quality and the like. In addition, product purity and the atom deuterated ratio are both 98%or above.