54604-57-8

Basic information

• Product Name: (22S)-22-Hydroxycholest-5-en-3β-yl Acetate
• Synonyms: (22S)-22-Hydroxycholest-5-en-3β-yl Acetate;WICQHKDZHVVRAU-MEMICBDASA-N
• CAS NO: 54604-57-8
• Molecular Formula: C29H48O3
• Molecular Weight: 444.68962
• Product Categories: Metabolites & Impurities, Pharmaceuticals, Intermediates & Fine Chemicals, Steroids
• Mol File: 54604-57-8.mol
• Article Data: 1

Chemical Properties

• Appearance: /
• Solubility: Chloroform, DCM, Ethyl Acetate
• CAS DataBase Reference: (22S)-22-Hydroxycholest-5-en-3β-yl Acetate(CAS DataBase Reference)
• NIST Chemistry Reference: (22S)-22-Hydroxycholest-5-en-3β-yl Acetate(54604-57-8)
• EPA Substance Registry System: (22S)-22-Hydroxycholest-5-en-3β-yl Acetate(54604-57-8)

Safety Data

• Hazardous Substances Data: 54604-57-8(Hazardous Substances Data)

Usage

Uses

(22S)-22-Hydroxycholest-5-en-3β-yl Acetate is an intermediate in the synthesis of 22S-metabolite of Cholesterol (C432501).

Upstream product

• 17711-16-9 22-(S)-hydroxycholesterol 
• 108-24-7 acetic anhydride 

Downstream Products

• 110536-31-7 22-fluorovitamin D₃ 
• 110536-30-6 22-fluoroprevitamin D₃ 

Relevant articles and documents

THE SYNTHESIS AND BIOLOGICAL ACTIVITY OF 22-FLUOROVITAMINE D3: A NEW VITAMIN D ANALOG

We synthesized 22-fluorovitamin D3 from (22S) cholest-5-ene-3β,22-diol-3β-acetate 2.Compound 2 was treated with diethylaminosulfur trifluoride to give 22-fluorocholest-5-en-3β-acetate 3 and (E) 22-dehydrocholest-5-en-3β-acetate.Compound 3 was treated with N-bromosuccinimide to give a mixture of the respective 5,7- and 4,6-dienes.The 5,7-diene of 3 was separated from the 4,6-diene using the dienophile 4-phenyl-1,2,4-triazoline-3,5-dione. 22-Fluoro-5α,8α-(3,5-dioxo-4-phenyl-1,2,4-triazolino)-cholest-6-en-3β-acetate 4 was purified by flash chromatography and treated with lithium aluminium hydride to generate 22-fluorocholesta-5,7-dien-3β-ol 5.Photolysis of the diene 5, followed by thermal equilibration, resulted in the synthesis of 22-fluorovitamin D3 7.The vitamin 7 increased active intestinal calcium transport only at a dose of 50,000 pmol/rat, whereas vitamin D3 increased intestinal calcium transport at a dose of between 50 and 500 pmol/rat. 22-Fluorovitamin D3 7 did not mobilize bone and soft tissue calcium at a dose as high as 50,000 pmol/rat, whereas vitamin D3 was successful in doing so at a dose of 500 pmol/rat.When tested in the duodenal organ culture system, 22-fluorovitamin D3 7 had approximately 1/25th the potency of vitamin D3.It did not antagonize the activity of 1,25-dihydroxyvitamin D3. 22-Fluorovitamin D3 7 bound to the rat plasma vitamin D binding protein less avidly than vitamin D3. 22-Fluorovitamin D3 was bound very poorly to the chick intestinal cytosol receptor for 1,25-dihydroxyvitamin D3.We conclude that the introduction of fluorine at the C-22 position results in a vitamin D sterol with decreased biologic activity when compared to vitamin D3.The presence of a fluorine group at C-22 position inhibits the binding of the vitamin to rat vitamin D binding protein when compared to the binding of its hydrogen analog, vitamin D3.