5541-93-5 Usage
Uses
Used in Enzyme Activity Assays:
2-PHOSPHOENOL PYRUVATE NA3SALT is used as a substrate in several enzyme activity assays for determining the kinetic parameters of different enzymes. It is particularly utilized in the following assays:
PRPP synthetase (PRPPS) assay
Ribokinase (RK) assay
Adenine phosphoribosyltransferase (APRT) assay
Used in Enzyme Kinetic Assays:
In the field of enzyme kinetics, 2-PHOSPHOENOL PYRUVATE NA3SALT is used as a component in the reaction mixture to determine the kinetic parameters of platelet-derived growth factor receptor α (PDGFRA). This helps researchers understand the enzyme's behavior and its role in various biological processes.
Used in ATP-Regenerating Systems:
When coupled with pyruvate kinase, 2-PHOSPHOENOL PYRUVATE NA3SALT completes an ATP-regenerating system. This system is essential for maintaining the energy charge within cells, as ATP is the primary energy currency in biological systems.
Used in Energy Charge Measurement:
As a component in buffer B, 2-PHOSPHOENOL PYRUVATE NA3SALT reacts with adenosine diphosphate (ADP) to form adenosine triphosphate (ATP). This reaction is crucial for energy charge measurement, which is an essential parameter in assessing the energy status of cells and tissues.
Biochem/physiol Actions
Phosphoenolpyruvic acid plays a major role in the initial step of glycolysis by acting as an inhibitor of hexokinase, phosphoglucose isomerase, phosphofructokinase and aldolase.
Check Digit Verification of cas no
The CAS Registry Mumber 5541-93-5 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 5,5,4 and 1 respectively; the second part has 2 digits, 9 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 5541-93:
(6*5)+(5*5)+(4*4)+(3*1)+(2*9)+(1*3)=95
95 % 10 = 5
So 5541-93-5 is a valid CAS Registry Number.
InChI:InChI=1/C3H5O6P.3Na/c1-2(3(4)5)9-10(6,7)8;;;/h1H2,(H,4,5)(H2,6,7,8);;;/q;3*+1/p-3
5541-93-5Relevant academic research and scientific papers
Silyl Phosphites. Part 20. A Facile Synthesis pf Phosphoenolpyruvate and Its Analogue Utilizing in siti Generated Trimethylsilyl Bromide
Sekine, Mitsuo,Futatsugi, Tetsuaki,Yamada, Kohji,Hata, Tsujiaki
, p. 2509 - 2514 (2007/10/02)
Phosphoenolpyruvate (PEP) has been synthesized from pyruvic acid and dimethyl trimethylsilyl phosphite in high yield by a new route which involves, successively, trimethylsilylation, bromination, and a Perkow reaction.A PEP analogue of 1-(dihydroxyphosphinyl)vinyl phosphate (1) has also been prepared.
A CONVENIENT SYNTHESIS OF PHOSPHOENOLPYRUVATE VIA SILYLESTER INTERMEDIATE
Sekine, Mitsuaki,Futatsugi, Tetsuaki,Yamada, Kohji,Hata, Tsujiaki
, p. 371 - 372 (2007/10/02)
Phosphoenolpyruvate was synthesized in high yield by a simple procedure starting from pyruvic acid and dimethyl trimethylsilyl phosphite through trimethylsililation, bromination, and the Perkow reaction.
