55466-22-3

Basic information

• Product Name: MUTANOLYSIN
• Synonyms: EC 3.2.1.17;N-ACETYLMURAMIDASE;N-ACETYLMURAMIDASE, STREPTOMYCES GLOBISPORUS;MUTANOLYSIN;MUTANOLYSIN ASSAY REAGENT;mutanolysin aseptically filled;mutanolysin F. streptomyces globisporus;Mutanolysin from Streptomyces globisporus ATCC?1553
• CAS NO: 55466-22-3
• Molecular Weight: 0
• Product Categories: Enzymes;Research Essentials;BacterialSignal Transduction Research Tools;Cell Lysis Reagents;EnzymesMolecular Biology;EnzymesResearch Essentials;Lysing Enzymes and Protoplast Preparation;Lysing EnzymesApplication Index;Cell Dissociation and Cell Lysis;Core Bioreagents;Enzymatic Cell Lysis and Protoplast Preparation;Enzymes, Inhibitors, and Substrates;Molecular Biology Enzymes
• Mol File: 55466-22-3.mol
• Article Data: 0

Chemical Properties

• Appearance: white/powder
• Storage Temp.: −20°C
• CAS DataBase Reference: MUTANOLYSIN(CAS DataBase Reference)
• NIST Chemistry Reference: MUTANOLYSIN(55466-22-3)
• EPA Substance Registry System: MUTANOLYSIN(55466-22-3)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 10-21
• Hazardous Substances Data: 55466-22-3(Hazardous Substances Data)

Usage

Description

Mutanolysin is an enzyme that has been used for various applications in the field of microbiology and molecular biology. It is known for its ability to provide gentle cell lysis, making it a valuable tool for the isolation of biomolecules and nucleic acids from bacterial cells.

Uses

Used in Microbiology Research:
Mutanolysin is used as a lysing agent for the generation of protoplasts from dairy streptococci and group B streptococci. This allows for the study of these bacteria and their properties, as well as the development of methods for their analysis and manipulation.
Used in Molecular Biology:
Mutanolysin is used for the gentle lysis of bacterial cells to isolate easily degradable biomolecules and RNA. This is particularly useful in research involving the study of gene expression and the identification of specific proteins and other biomolecules.
Used in DNA Isolation:
Mutanolysin is used in the formation of spheroplasts, which are a type of bacterial cell that has had its cell wall partially or completely removed. This process is important for the isolation of DNA from bacterial cells, as it allows for the efficient extraction of genetic material for further analysis and manipulation.

Biochem/physiol Actions

Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.