55487-93-9

Usage

Uses

Used in Research Applications:
4-METHYLUMBELLIFERYL-BETA-D-FUCOPYRANOSIDE is used as a research tool for the detection and quantification of β-D-Fucosidase enzyme activity. The reason for its application is that it provides a sensitive and reliable method to measure the enzyme's activity, which is crucial in understanding the enzyme's role in various biological processes.
Used in Diagnostic Applications:
In the field of diagnostics, 4-METHYLUMBELLIFERYL-BETA-D-FUCOPYRANOSIDE is used as a diagnostic marker for certain diseases and conditions. The application reason is that the enzyme β-D-Fucosidase is involved in the metabolism of various substances, and its abnormal activity can be indicative of specific health issues. By measuring the enzyme's activity using this substrate, medical professionals can gain valuable insights into a patient's condition and guide appropriate treatment.
Used in Pharmaceutical Industry:
4-METHYLUMBELLIFERYL-BETA-D-FUCOPYRANOSIDE is used as a quality control agent in the pharmaceutical industry for the assessment of β-D-Fucosidase inhibitors. The application reason is that these inhibitors can be potential therapeutic agents for treating diseases associated with abnormal β-D-Fucosidase activity. By using this substrate, researchers can evaluate the effectiveness of these inhibitors and optimize their development for clinical use.
Used in Biotechnology Applications:
In the biotechnology industry, 4-METHYLUMBELLIFERYL-BETA-D-FUCOPYRANOSIDE is used as a substrate for the development of biosensors and high-throughput screening assays. The application reason is that the blue fluorescent signal produced upon enzyme cleavage allows for the rapid and accurate detection of β-D-Fucosidase activity, which can be integrated into various biotechnological applications, such as drug discovery and enzyme engineering.

InChI:InChI=1/C16H18O7/c1-7-5-12(17)23-11-6-9(3-4-10(7)11)22-16-15(20)14(19)13(18)8(2)21-16/h3-6,8,13-16,18-20H,1-2H3/t8-,13+,14+,15-,16+/m1/s1

Upstream product

• 16741-27-8 2,3,4-tri-O-acetyl-α-L-fucopyranosyl bromide 
• 90-33-5 7-hydroxy-4-methyl-chromen-2-one 
• 106488-00-0 C₂₂H₂₄O₁₀ 
• 7404-35-5 1,2,3,4-tetra-O-acetyl-L-rhamnopyranose 
• 28915-80-2 2,3,4-tri-O-acetyl-L-rhamnopyranose 
• 122089-71-8 2,3,4-tri-O-acetyl-L-rhamnopyranosyl trichloroacetimidate 

Relevant academic research and scientific papers

CARBONIC ANHYDRASE INHIBITORS WITH ANTIMETASTATIC ACTIVITY

Compositions for the treatment of cancer comprising coumarin and thiocoumarin derivatives of Formulas I- XII are disclosed. Said derivatives preferentially inhibit carbonic anhydrase IX and XII (which are associated with hypoxic and metastatic tumours) over inhibiting carbonic anhydrase I and II activity. The compositions therefore are suited for treatment of hypoxic or metastatic cancers due to this selective mechanism of action.

Glycosyl coumarin carbonic anhydrase IX and XII inhibitors strongly attenuate the growth of primary breast tumors

A series of 7-substituted coumarins incorporating various glycosyl moieties were synthesized and investigated for the inhibition of the zinc enzyme carbonic anhydrase (CA, EC 4.2.1.1). These coumarins were very weak or ineffective as inhibitors of the housekeeping, offtarget isoforms CA I and II, but some of them inhibited tumor-associated CA IX and XII in the low nanomolar range. They also significantly inhibited the growth of primary tumors by the highly aggressive 4T1 syngeneic mouse mammary tumor cells at 30 mg/kg, constituting interesting candidates for the development of conceptually novel anticancer drugs. Because CA IX is overexpressed in hypoxic tumors and exhibits very limited expression in normal tissues, such compounds may be useful for treating cancers not responsive to classic chemo- and radiotherapy.

Profiling of glycosidase activities using coumarin-conjugated glycoside cocktails

Glycosidases are a large subgroup of carbohydrate-processing enzymes that hydrolytically cleave the glycosidic bond. Glycans formed by the action of glycosidases are involved in various biological processes. Genetic abnormalities in glycosidases are associated with inherited diseases. Thus, characterization of the catalytic activities of glycosidases is of great importance. Herein, we describe a simple and rapid approach for determining glycosidase activity profiles using coumarin-conjugated glycoside cocktails.