5725-89-3 Usage
Uses
Used in Cytochrome P-450 Differentiation:
Methoxyresorufin is used as a substrate for differentiating isozymes of cytochrome P-450. It aids in identifying and distinguishing between various isoforms of the enzyme, which play a crucial role in drug metabolism and detoxification processes.
Used in Cytochrome P-450 Activity Quantification:
Methoxyresorufin serves as a substrate for quantifying the activity of cytochrome P450 isoforms, particularly CYP1A1 and CYP1A2. The released resorufin's fluorescence can be measured, providing a reliable method to assess the enzyme's activity levels in various samples.
Used in Drug Metabolism Studies:
In the pharmaceutical industry, Methoxyresorufin is used as a tool to study the metabolism of drugs and other xenobiotics. By monitoring the activity of specific cytochrome P450 isoforms, researchers can gain insights into the metabolic pathways and potential drug-drug interactions.
Used in Environmental Toxicology:
Methoxyresorufin is employed in environmental toxicology to assess the impact of pollutants and toxins on the cytochrome P450 system. This helps in understanding the potential health risks associated with exposure to various environmental contaminants.
Used in High-Performance Liquid Chromatography (HPLC):
In analytical chemistry, Methoxyresorufin is used as a substrate in the incubation mixture for determining cytochrome P4501A activities, such as ethoxyresorufin O-deethylase (EROD) and methoxyresorufin O-demethylase (MROD) in liver microsomes. The HPLC technique allows for accurate measurement and analysis of these activities.
Biochem/physiol Actions
Fluorimetric substrate for cytochrome P450 linked enzymes.
Check Digit Verification of cas no
The CAS Registry Mumber 5725-89-3 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 5,7,2 and 5 respectively; the second part has 2 digits, 8 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 5725-89:
(6*5)+(5*7)+(4*2)+(3*5)+(2*8)+(1*9)=113
113 % 10 = 3
So 5725-89-3 is a valid CAS Registry Number.
InChI:InChI=1/C13H9NO3/c1-16-9-3-5-11-13(7-9)17-12-6-8(15)2-4-10(12)14-11/h2-7H,1H3
5725-89-3Relevant academic research and scientific papers
Regioselective Difluoromethane sulfonylation and Triflylation of Resorufin Derivatives
Tan, Qingwei,Zhao, Shuxuan,Li, Yuyao,Jiang, Jialing,Tang, Huiling,Chen, Yefeng,Peng, Yan,Xie, Hexin
supporting information, p. 8477 - 8481 (2021/11/13)
Reported herein is a regioselective difluoromethane sulfonylation or triflylation of resorufin derivatives, which allows easy access to 2-difluoromethane sulfonylated or triflylated resorufin derivatives in good yields. The installation of a difluorometha
