57360-69-7 Usage
Uses
MTT Formazan has been used in cell viability assay.
Biochem/physiol Actions
MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.
Check Digit Verification of cas no
The CAS Registry Mumber 57360-69-7 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 5,7,3,6 and 0 respectively; the second part has 2 digits, 6 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 57360-69:
(7*5)+(6*7)+(5*3)+(4*6)+(3*0)+(2*6)+(1*9)=137
137 % 10 = 7
So 57360-69-7 is a valid CAS Registry Number.
InChI:InChI=1/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+