57407-08-6

Basic information

• Product Name: DEAE SEPHAROSE CL-6B
• Synonyms: DEAE SEPHAROSE CL-6B;DEAE SEPHAROSE FAST FLOW;DEAE-SEPHAROSE(R) CL-6B;DIETHYLAMINOETHYL SEPHAROSE;DIETHYLAMINOETHYL-SEPHAROSE(R) CL-6B;agarose,2-(diethylamino)ethylether;deae sepharose anion exchanger;deae-sepharose(R) 'fast flow'
• CAS NO: 57407-08-6
• Molecular Weight: 0
• Product Categories: AMEKO
• Mol File: 57407-08-6.mol
• Article Data: 0

Chemical Properties

• Flash Point: 44 °C
• Appearance: /
• Storage Temp.: 2-8°C
• CAS DataBase Reference: DEAE SEPHAROSE CL-6B(CAS DataBase Reference)
• NIST Chemistry Reference: DEAE SEPHAROSE CL-6B(57407-08-6)
• EPA Substance Registry System: DEAE SEPHAROSE CL-6B(57407-08-6)

Safety Data

• Hazard Codes: Xi
• Statements: 10-36/37/38
• Safety Statements: 16-26-36
• RIDADR: UN 1170 3/PG 3
• WGK Germany: 1
• F: 10
• Hazardous Substances Data: 57407-08-6(Hazardous Substances Data)

Usage

Description

DEAE SEPHAROSE CL-6B is an anion exchange chromatography medium, which is a type of gel used for the purification of proteins and other biomolecules. It is a highly cross-linked agarose matrix with quaternary ammonium groups that provide a strong anion exchange capacity. This medium is widely used in various applications due to its high capacity, excellent flow properties, and ability to bind a wide range of biomolecules.

Uses

Used in Biochemistry and Molecular Biology:
DEAE SEPHAROSE CL-6B is used as an anion exchange chromatography medium for the purification of proteins, nucleic acids, and other biomolecules. It is particularly useful for separating molecules based on their charge properties, allowing for the isolation of specific proteins or other biomolecules from complex mixtures.
Used in Pharmaceutical Industry:
DEAE SEPHAROSE CL-6B is used as a purification agent for the production of therapeutic proteins and other biopharmaceuticals. Its high capacity and selectivity make it an ideal choice for the large-scale purification of proteins, which is crucial for the development and manufacturing of various drugs.
Used in Diagnostics:
DEAE SEPHAROSE CL-6B is used as a key component in the development of diagnostic assays, particularly for the detection of toxins produced by pathogenic strains of bacteria, such as Clostridium difficile. Its ability to selectively bind and separate target molecules makes it a valuable tool in the development of sensitive and specific diagnostic tests.
Used in Research and Development:
DEAE SEPHAROSE CL-6B is used as a research tool for studying the pathogenesis of human diseases and for the development of new therapies. Its versatility in separating and purifying biomolecules makes it an essential tool in the hands of researchers working on various aspects of human health and disease.
Used in Anion Exchange Chromatography:
DEAE SEPHAROSE CL-6B is used as a stationary phase in anion exchange chromatography for the separation and purification of negatively charged molecules, such as proteins, nucleic acids, and other biomolecules. Its high capacity and selectivity make it an ideal choice for a wide range of applications in biochemistry, molecular biology, and biotechnology.
Used in Affinity Chromatography:
DEAE SEPHAROSE CL-6B can also be used in affinity chromatography, where it can be functionalized with specific ligands to selectively bind and purify target molecules. This application allows for the highly specific and efficient purification of proteins and other biomolecules, which is particularly useful in the development of new drugs and therapies.
Used in Protein Chromatography:
DEAE SEPHAROSE CL-6B is used as a matrix in protein chromatography for the separation and purification of proteins based on their charge properties. Its high capacity and excellent flow properties make it an ideal choice for the large-scale purification of proteins, which is essential for various applications in the pharmaceutical, biotechnology, and research industries.
Used in Ion Exchange Chromatography:
DEAE SEPHAROSE CL-6B is used as a matrix in ion exchange chromatography for the separation and purification of charged molecules, such as proteins, peptides, and nucleic acids. Its ability to selectively bind and separate molecules based on their charge properties makes it a valuable tool in the development of new drugs, therapies, and diagnostic assays.

Biochem/physiol Actions

Diethylaminoethyl-sepharose (DEAE-sepharose) is a strong anion exchange column, where DEAE covalently binds to sepharose.