578021-02-0Relevant articles and documents
Detection of Localized Hepatocellular Amino Acid Kinetics by using Mass Spectrometry Imaging of Stable Isotopes
Arts, Martijn,Soons, Zita,Ellis, Shane R.,Pierzchalski, Keely A.,Balluff, Benjamin,Eijkel, Gert B.,Dubois, Ludwig J.,Lieuwes, Natasja G.,Agten, Stijn M.,Hackeng, Tilman M.,van Loon, Luc J. C.,Heeren, Ron M. A.,Olde Damink, Steven W. M.
supporting information, p. 7146 - 7150 (2017/06/13)
Mass spectrometry imaging (MSI) simultaneously detects and identifies the spatial distribution of numerous molecules throughout tissues. Currently, MSI is limited to providing a static and ex vivo snapshot of highly dynamic systems in which molecules are constantly synthesized and consumed. Herein, we demonstrate an innovative MSI methodology to study dynamic molecular changes of amino acids within biological tissues by measuring the dilution and conversion of stable isotopes in a mouse model. We evaluate the method specifically on hepatocellular metabolism of the essential amino acid l-phenylalanine, associated with liver diseases. Crucially, the method reveals the localized dynamics of l-phenylalanine metabolism, including its in vivo hydroxylation to l-tyrosine and co-localization with other liver metabolites in a time course of samples from different animals. This method thus enables the dynamics of localized biochemical synthesis to be studied directly from biological tissues.
METHOD AND REAGENT FOR ANALYSIS OF AMINO FUNCTIONAL COMPOUND
-
, (2016/10/27)
PROBLEM TO BE SOLVED: To provide a method for easily analyzing with high sensitivity amino functional compounds such as amino acids or peptides contained in a sample. SOLUTION: Amino functional compounds in a sample containing the amino functional compoun
Multifunctional and highly sensitive precolumn reagents for amino acids in liquid chromatography/tandem mass spectrometry
Shimbo, Kazutaka,Yahashi, Akihisa,Hirayama, Kazuo,Nakazawa, Masakazu,Miyano, Hiroshi
experimental part, p. 5172 - 5179 (2010/04/04)
We have developed novel precolumn derivatization reagent, p-N,N,N-trimethylammonioanilyl N′-hydroxysuccinimidyl carbamate iodide (TAHS), for sensitive analyses of amino acids using high-performance liquid chromatography/electrospray ionization tandem mass