5893-78-7Relevant academic research and scientific papers
Synthesis of oligonucleotide-peptide conjugates containing a KDEL signal sequence
Arar,Monsigny,Mayer
, p. 8087 - 8090 (2007/10/02)
An improved method of preparation of oligonucleotide-peptide conjugates is described. An oligopeptide containing α and ε-aminogroups is mainly substituted at its α-NH2 end by ε-maleimidocaproic acid-N-hydroxysuccinimide ester at pH 6.5 for 1 h. The N(α)-maleimidocaproyl-peptide derivative, purified by HPLC, reacts with the thiol group of an oligonucleotide in a 82% yield at pH 7.2. The thiol group is generated in situ by the action of tris(carboxyethyl)phosphine on an oligonucleotide bearing a disulfide bridge.
