Z-Thr-ONB, also known as N-benzyloxycarbonyl-L-threonine p-nitrobenzyl ester, is a synthetic peptide compound that serves as a substrate for various enzymes, particularly proteases. It is a derivative of L-threonine, an essential amino acid, with a benzyloxycarbonyl (Z) protecting group and a p-nitrobenzyl (ONB) ester group. The Z group protects the amino group, while the ONB group is sensitive to enzymatic hydrolysis, making it a useful tool for studying enzyme kinetics and activity. Upon enzymatic cleavage, the ONB group is released, and its absorbance at 410 nm can be measured, allowing for the quantification of enzyme activity. Z-Thr-ONB is widely used in research and diagnostic applications to assess the functionality of proteolytic enzymes and to screen for potential enzyme inhibitors.
The CAS Registry Mumber 58932-19-7 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 5,8,9,3 and 2 respectively; the second part has 2 digits, 1 and 9 respectively. Calculate Digit Verification of CAS Registry Number 58932-19: (7*5)+(6*8)+(5*9)+(4*3)+(3*2)+(2*1)+(1*9)=157 157 % 10 = 7 So 58932-19-7 is a valid CAS Registry Number.