5959-90-0Relevant articles and documents
Isolation and identification of diadenosine 5′,5?-P 1,P4-tetraphosphate binding proteins using magnetic bio-panning
Guo, Wei,Azhar, M. Ameruddin,Xu, Yuhong,Wright, Michael,Kamal, Ahmed,Miller, Andrew D.
experimental part, p. 7175 - 7179 (2012/02/02)
We report the development of a synthetic, biotin-conjugated diadenosine tetraphosphate (Ap4A)-'molecular hook' attached to magnetic beads enabling the isolation of Ap4A-binding proteins from bacterial cells or mammalian tissue lysates. Characterisation and identification of isolated binding proteins is performed sequentially by mass spectrometry. The observation of positive controls suggests that these newly observed proteins are putative Ap4A-binding partners, and we have expectations that others can be found with further technical improvements in our methods.
NEW USES OF DINUCLEOTIDE POLYPHOSPHATE DERIVATIVES
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Page/Page column 11, (2008/06/13)
The present invention provides the use of analogues and derivatives of dinucleoside polyphosphates with formula (I) or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for use in one or more of: the treatment of ischemia, inducing ischemic tolerance, modulating cerebral ischemia, to delay the onset of a hypoxic depolarisation stage when ischemic events are initiated; as a neurological protection agent; as a tissue protection agent; the treatment of pain; and the treatment of inflammation, wherein X, is selected from wherein X1 and X2 are independently selected from H, Cl, Br and F; each Y is independently selected from S and O; each Z is independently selected from -CX3X4-,-NH-,-O- ; wherein X3 and X4 are selected from H, CI, Br and F; B1 and B2 are independently selected from adenine, guanine, xanthine, thymine, uracil, cytosine and inosine; S1 and S2 are independently selected from ribose, open chain ribose, 2'-deoxyribose, 3'deoxyribose and arabinofuranoside. V is selected from 0, 1 , 2, 3, 4 and 5; W is selected from 0, 1 , 2, 3, 4 and 5; and V plus W is an integer from 2 to 6.
Synthesis and reactions of nucleoside 5'-diphosphate imidazolide. A nonenzymatic capping agent for 5'-monophosphorylated oligoribonucleotides in aqueous solution
Sawai, Hiroaki,Wakai, Hiromichi,Nakamura-Ozaki, Akiko
, p. 5836 - 5840 (2007/10/03)
We have synthesized adenosine and 7-methylguanosine 5'-diphosphate imidazolides from imidazole and the corresponding nucleoside 5'-diphosphates. The phosphorimidazolide bond of the compounds was susceptible to hydrolysis and hydrolyzed gradually in neutral aqueous solution, but it was more stable than that of the corresponding imidazolides of nucleoside 5'-monophosphate. The 7-methylguanosine 5'-diphosphate imidazolide reacted with guanosine 5'- monophosphate or 5' monophosphorylated oligoribonucleotides in neutral aqueous solution in the presence of an Mn2- ion catalyst converting to the cap portion of mRNA or the capped m7Gppp-oligoribonucleotides in substantial yields. The condensation reaction of adenosine 5'-diphosphate imidazolide with adenosine 5'-monophosphate took place similarly in neutral aqueous solution by a divalent metal ion-catalyst such as Mg2+ or Mn2+, yielding diadenosine 5',5'-triphosphate.