5972-45-2Relevant academic research and scientific papers
Safe and efficient in vitro and in vivo gene delivery: Tripodal cationic lipids with programmed biodegradability
Unciti-Broceta, Asier,Moggio, Loredana,Dhaliwal, Kevin,Pidgeon, Laura,Finlayson, Keith,Haslett, Chris,Bradley, Mark
, p. 2154 - 2158 (2011)
The therapeutic use of nucleic acids has long been heralded as a panacea of medicinal opportunity, a vision enhanced by the introduction of RNA interference technology. The Achilles heel of such an approach is the in vivo delivery of the desired nucleic acid into cells, a practice that lacks selectivity, safety and/or efficiency. Herein we report the safe and efficacious in vitro and in vivo delivery of nucleic acids using tripodal biodegradable cationic lipids. Toxicity reduction and transfection potency of these novel amphiphiles were addressed by designing the compounds to undergo complete intracellular degradation thereby enhancing cargo release while minimising toxicity and potential tissue accumulation. Compounds demonstrated high-efficiency in transfecting DNA into cells both in vitro and in vivo with no signs of toxicity, thus potentially offering a safer alternative to viral transfection for gene therapy application. The Royal Society of Chemistry.
Design and synthesis of lipids for the fabrication of functional lipidic cubic-phase biomaterials
Osornio, Yazmin M.,Uebelhart, Peter,Bosshard, Silvan,Konrad, Fabian,Siegel, Jay S.,Landau, Ehud M.
, p. 10583 - 10595 (2013/02/22)
A series of novel lipids with designed functionalities were synthesized. These lipids are based on conjugation of α-amino acids and their esters, cationic, anionic, neutral, and photochromic moieties to the lipophilic 9-cis octadecenyl chains by amide, ester, thioester, or amine bonds. Because of the plasticity of lipidic cubic phases, it is envisaged that when mixed with monooleoyl-rac-glycerol (monoolein, MO) and water at appropriate proportions, they would assemble to form bicontinuous lipidic cubic phases (LCPs) that exhibit the well-known material properties of LCPs such as phase stability, optical transparency, and chemical permeability. Moreover, due to the nature and position of the functionality at the headgroup region, we envision them to perform as functional materials by design.
CATIONIC LIPIDS
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Page/Page column 27, (2009/05/28)
This invention relates to cationic lipids. More particularly the invention relates to biodegradable cationic lipids having a plurality of cationic headgroups and one or more lipophilic tail groups. The lipids are of utility in various applications, and in particular in permitting transfection of molecules, and in particular DNA and RNA, into cells. As such the lipids have specific utility in the field of gene therapy as well as other applications such as delivery of small molecules into cells, detergents, and metal ion complexation for medical or industrial applications.
