599-07-5Relevant articles and documents
Medicagenic acid saponins from Aster batangensis
Shao Yu,Zhou Bingnan,Ma Kan,Wu Houming,Lin Longze,Cordell
, p. 875 - 881 (1995)
-
Two new compounds from Semen celosiae and their protective effects against CCl4-induced hepatotoxicity
Xue, Qian,Sun, Zhen-Liang,Guo, Mei-Li,Wang, Ying,Zhang, Ge,Wang, Xiao-Kang
body text, p. 772 - 780 (2011/06/17)
Two new oleanolic acid saponins, namely celosin A (1) and celosin B (2), together with six known compounds, stigmasterol (3), β-sitosterol (4),-daucosterol (5), hexacosoic acid (6), palmitic acid (7) and stearic acid (8), were isolated from the ethanolic extract of Semen celosiae. The structures of celosin A (1) and celosin B (2) were determined by spectral analysis (including 1D-and 2D-NMR). The hepatoprotective activity of 1 and 2 with oral doses 1.0, 2.0 and 4.0 mg kg-1 were investigated by carbon tetrachloride (CCl4)-induced hepatotoxicity in mice. The results indicate that they have significant hepatoprotective effects, and that these hepatoprotective effects may be due to the antioxidant capability.
STRUCTURAL ELUCIDATION OF ALFALFA ROOT SAPONINS BY MASS SPECTROMETRY AND NUCLEAR MAGNETIC RESONANCE ANALYSIS
Massiot, Georges,Lavaud, Catherine,Men-Olivier, Louisette Le,Binst, Georges Van,Miller, Stephen P. F.,Fales, Henry M.
, p. 3071 - 3080 (2007/10/02)
Structures of saponins may be fully elucidated using a combination of n.m.r. and m.s. instead of the usual degradation techniques.Well resolved proton n.m.r. spectra and chromatographic separation can be obtained from peracetylated mixtures of saponins.Under these conditions, genins are identified mainly by (13)C n.m.r. spectroscopy; sugars and their points of attachment are determined by examining (1)H chemical shifts and coupling constants following assignments made by 2D COSY and relayed COSY experiments.Sugar chains are sequenced by observing intersugar connectivities through long-range couplings (delayed COSY) or n.O.e.Alternatively, the use of Californium plasma desorption m.s. provides quasimolecular ions and fragments corresponding to the sequential rupture of the chain of sugars.Free carboxylic acids are located on an acetylated saponin by looking at the disappearance, in the presence of Eu complexes, of the carbon situated α to the carbonyl in the (13)C n.m.r. spectrum.The above methods have been employed to determine the structures of the seven major saponins of alfalfa root, which are allegedly responsible for the antifeedant properties of the vegetable.
