602-52-8

Usage

Uses

Used in Biomedical Research:
3,8-diamino-5-ethyl-6-phenylphenanthridinium chloride is used as a fluorescent staining agent for nucleic acids, such as DNA and RNA, in various applications like gel electrophoresis, fluorescence microscopy, and flow cytometry. Its ability to bind to nucleic acids and emit fluorescence allows researchers to study the structure, function, and interactions of these molecules.
Used in Clinical Diagnostics:
In the clinical diagnostics industry, 3,8-diamino-5-ethyl-6-phenylphenanthridinium chloride is used as a fluorescent marker for detecting and quantifying nucleic acids in samples. Its high sensitivity and specificity make it suitable for diagnosing various diseases, including genetic disorders and infectious agents, by analyzing the presence and quantity of specific nucleic acid sequences.
Used in Cancer Research:
3,8-diamino-5-ethyl-6-phenylphenanthridinium chloride has been studied as a potential anti-cancer agent due to its ability to bind to DNA and interfere with cell division. However, its use as a therapeutic agent is limited due to its potential cytotoxicity and lack of selectivity towards cancer cells. Further research is needed to explore its potential applications in cancer treatment while minimizing side effects.

InChI:InChI=1/C21H19N3.ClH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H

Synthetic route

C30H27N4O3(1+)*H2O4P(1-) → [3H]-Ethidium (602-52-8) + 3-urea-ethidium chloride

Conditions	Yield
With hydrogenchloride; water In methanol at 96℃; for 1h;

C30H27N4O3(1+)*H2O4P(1-) → [3H]-Ethidium (602-52-8) + 8-urea-ethidium chloride

Conditions	Yield
With hydrogenchloride; water In methanol at 96℃; for 1h;

[3H]-Ethidium (602-52-8) + 3-amino-benzamidine hydrochloride → 5-ethyl-3,8-diamino-7-(3-carbamimidoyl-phenylazo)-6-phenyl-phenanthridinium; chloride-hydrochloride + 5-ethyl-3-amino-8--6-phenyl-phenanthridinium chloride-hydrochloride

Conditions	Yield
With hydrogenchloride; sodium acetate; sodium nitrite

[3H]-Ethidium (602-52-8) + 4-amino-benzamidine hydrochloride → 5-ethyl-3,8-diamino-7-(4-carbamimidoyl-phenylazo)-6-phenyl-phenanthridinium; chloride-hydrochloride + 5-ethyl-3-amino-8--6-phenyl-phenanthridinium chloride-hydrochloride

Conditions	Yield
With hydrogenchloride; sodium acetate; sodium nitrite

[3H]-Ethidium (602-52-8) + 3-amino-benzamidine hydrochloride → 5-ethyl-3-amino-8-[N'-(3-carbamimidoyl-phenyl)-triazenyl]-6-phenyl-phenanthridinium; chloride-hydrochloride (6798-24-9) + 5-ethyl-3,8-diamino-7(or 9)-<3-carbamimidoyl-phenylazo>-6-phenyl-phenanthridinium chloride-hydrochloride

Conditions	Yield
With hydrogenchloride; sodium acetate; sodium nitrite

[3H]-Ethidium (602-52-8) + 4-amino-benzamidine hydrochloride → 5-ethyl-3-amino-8-[N'-(4-carbamimidoyl-phenyl)-triazenyl]-6-phenyl-phenanthridinium; chloride-hydrochloride (4174-67-8) + 5-ethyl-3,8-diamino-7(or 9)-<4-carbamimidoyl-phenylazo>-6-phenyl-phenanthridinium chloride-hydrochloride

Conditions	Yield
With hydrogenchloride; sodium acetate; sodium nitrite

Downstream Products

• 6798-24-9 5-ethyl-3-amino-8-[N'-(3-carbamimidoyl-phenyl)-triazenyl]-6-phenyl-phenanthridinium; chloride-hydrochloride 
• 4174-67-8 5-ethyl-3-amino-8-[N'-(4-carbamimidoyl-phenyl)-triazenyl]-6-phenyl-phenanthridinium; chloride-hydrochloride 

Relevant academic research and scientific papers

NOVEL ANTI-VIRAL AGENTS BASED UPON DERIVATIVES OF THE AROMATIC HETEROCYCLE PHENANTHRIDINE

A series of substituted phenanthridine derivatives has been synthesized by converting the amines at the 3- and 8- positions of ethidium bromide into guanidine, pyrrole, urea, and various substituted ureas. The resulting derivatives exhibit unique spectral properties that change upon binding nucleic acids. The compounds have an enhanced affinity and specificity for HIV-1 Rev Response Element as compared to ethidium bromide.