6104-58-1 Usage
Description
Acid Blue 90, also known as Brilliant Blue G, is a synthetic azo dye that is soluble in water and exhibits a blue color. It is commonly used in various applications across different industries due to its staining properties and ability to bind with proteins.
Uses
Used in Analytical Biochemistry:
Acid Blue 90 is used as a dye in analytical biochemistry for staining proteins, allowing for the visualization of protein bands during gel electrophoresis. It is particularly useful for SDS gels, as it readily stains proteins with minimal background color.
Used in Protein Assays:
Acid Blue 90 is used as a protein reagent in the Bradford dye-binding protein assay, which is a method for determining protein content. A mechanism for dye binding to protein has been proposed based on measuring the absorbance spectra of Coomassie Brilliant Blue G-250 during titration of the dye reagent in the absence of protein and its response to different polyamino acids.
Used in Pharmaceutical Applications:
Acid Blue 90 is used as a P2X7 antagonist, which is a type of receptor found on immune cells. Antagonizing this receptor can have potential therapeutic effects in various conditions.
Used in Surgical Applications:
Acid Blue 90 may be employed as a stain for the internal limiting membrane (ILM) during macular hole (MH) and epiretinal membrane (ERM) surgery, aiding in the visualization and removal of these membranes.
Used in Fish Waste Processing:
Acid Blue 90 is used to prepare the protein reagent for the determination of protein content of the collagenase enzyme isolated from fish waste, which can be utilized in various applications such as the production of gelatin or as a biocatalyst in the food industry.
Used in Detergent Analysis:
Acid Blue 90 is useful for determining the critical micelle concentration of detergents, which is an important parameter in understanding the behavior and effectiveness of detergent formulations.
Preparation
Benzaldehyde ?(1 Moore) and N-ethyl-N-benzyl-3-methylaniline?(2 Moore) condensation, And then sulfonating the product of three acids, and lead oxide, peroxide, and then the reaction with the 4-Ethoxybenzenamine, Benzaldehyde ?sulfonic acid groups on the phenyl ring is substituted anilino-ethoxy.
Flammability and Explosibility
Notclassified
Biological Activity
brilliant blue g is also a selective inhibitor of the p2x purinoceptor channel p2x7 [1].p2x receptors are membrane ion channels activated in response to the binding of extracellular atp. seven p2x subtypes have been identified. p2x receptors have been involved in in diverse patho- and physiological processes, such as the autonomic nervous system, afferent signalling, chronic pain, and in autocrine loops of endothelial and epithelial cells. the p2x7 receptor plays a prominent role in certain neurologic disorders, such as ischemia-reperfusion injury, alzheimer's disease, spinal cord injury and sensory neuropathies [2].in hek293 cells heterologously expressing human p2x7 receptors, brilliant blue g noncompetitively inhibited rat and human p2x7 receptors with ic50 values of 10 and 200 nm, respectively. the ic50 values for inhibition of the other p2x receptors ranged from 2 to >30 μm; brilliant blue g inhibited the rat and human p2x4 receptors with the ic50 values of >10 and 3.2 μm [1]. subretinal injection of bbg caused retinal cell degeneration at lower concentrations. subretinal injection of bbg (0.25 mg/ml) provided satisfactory biocompatibility [3].
Biochem/physiol Actions
P2X7 purinergic receptor antagonist.
Standard
Light Fastness
Fading
Stain
ISO
3
AATCC
3
references
[1] jiang l h, mackenzie a b, north r a, et al. brilliant blue g selectively blocks atp-gated rat p2x7 receptors[j]. molecular pharmacology, 2000, 58(1): 82-88.[2] sperlágh b, vizi e s, wirkner k, et al. p2x 7 receptors in the nervous system[j]. progress in neurobiology, 2006, 78(6): 327-346.[3] ueno a, hisatomi t, enaida h, et al. biocompatibility of brilliant blue g in a rat model of subretinal injection[j]. retina, 2007, 27(4): 499-504.
Check Digit Verification of cas no
The CAS Registry Mumber 6104-58-1 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 6,1,0 and 4 respectively; the second part has 2 digits, 5 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 6104-58:
(6*6)+(5*1)+(4*0)+(3*4)+(2*5)+(1*8)=71
71 % 10 = 1
So 6104-58-1 is a valid CAS Registry Number.
InChI:InChI=1/C47H49N3O7S2/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56/h9-30H,6-8,31-32H2,1-5H3,(H2,51,52,53,54,55,56)/p+1