61278-06-6Relevant articles and documents
Is sulfate lost during the chemical release of oligosaccharides from glycoproteins?
King, Kevin R.,Williams, J. Michael,Clamp, John R.,Corfield, Anthony P.
, p. 41 - 49 (1996)
Model experiments using methyl α-D-galactopyranoside 6-sulfate have implied that the Carlson conditions (1 M sodium borohydride and 0.05 M sodium hydroxide at 45 °C) for the release of O-linked oligosaccharides from glycoproteins do not cause significant loss of sulfate ester groups. However the more vigorous conditions used to release N-linked oligosaccharides are more likely to cause considerable sulfate loss from a galactoside 6-sulfate as a result of 3,6-anhydride formation. Experiments with dextran sulfate suggested that sulfate loss due to oxirane formation was also not significant under the Carlson conditions. These results were supported by experiments with [35S]sulfate-labelled human rectal mucus glycoprotein.
