61596-38-1Relevant academic research and scientific papers
Rational design, synthesis and biological evaluation of modular fluorogenic substrates with high affinity and selectivity for PTP1B
Sanchini, Silvano,Perruccio, Francesca,Piizzi, Grazia
, p. 961 - 976 (2014/05/20)
Protein-tyrosine phosphatase 1B (PTP1B) is a key regulatory enzyme in several signal transduction pathways, and its upregulation has been associated with type-2 diabetes, obesity and cancer. Selective determination of the functional significance of PTP1B
Mechanism and Mechanism-Based Inactivation of 4-Hydroxyphenylpyruvate Dioxygenase
Forbes, Brian J.R.,Hamilton, Gordon A.
, p. 343 - 361 (2007/10/02)
Six substrate analogs of 4-hydroxyphenylpyruvate, specifically pentafluorophenylpyruvate, 4-hydroxytetrafluorophenylpyruvate, 2-thienylpyruvate, 3-thienylpyruvate, thiophenol oxalate, and p-thiocresol oxalate were synthesized and their interactions with porcine liver 4-hydroxyphenylpyruvate dioxygenase investigated.Both pentafluorophenylpyruvate and thiophenol oxalate are competitive inhibitors of the enzyme with K1 values of 14 and 150 μM, respectively, but p-thiocresol oxalate has no effect on the enzymic activity.The other three substrate analogs are both substrates and mechanism-based inactivators of the enzyme with the following kinetic characteristics (compound, Km, Vmax,kinact, K', partition ratio) at pH 6.0, 37 deg C, and an air atmosphere: 4-hydroxytetrafluorophenylpyruvate, 50 μM, 1.9 mkat/kg, 1.5/min, 70 μM, 4.2; 2-thienylpyruvate, 500μM, 7.8 mkat/kg, 0.6/min, 400 μM, 41; 3-thienylpyruvate, 250 μM, 2.9 mkat/kg, 0.6/min, 300 μM, 22.When inactivated, the dioxygenase was found to contain per mole of active enzyme, 0.78 mol of label from 3-thienyl-3pyruvate and 0.85 mol of label from 4-hydroxytetrafluorophenyl-3pyruvate.The product formed from the enzyme-catalyzed oxidation of 3-thienylpyruvate was determined to be 3-carboxymethyl-3-thiolene-2-one.The implication of these results to the mechanism of the dioxygenase is considered.
Reactions of Polyfluoro-arenols and -heteroarenols with Activated Dimethyl Sulphoxide. Facile -Sigmatropic Rearrangement Reactions giving De-aromatised Products
Brooke, Gerald M.,Ferguson, J. A. K. Jamie
, p. 2091 - 2098 (2007/10/02)
Pentafluorophenol reacted with the reagent dimethyl sulphoxide--dicyclohexylcarbodi-imide--orthophosphoric acid below room temperature to give the ether (1), the -rearrangement product (2), and (3) a derivative of (2).Under similar conditions, 2,3,5,6-tetrafluorophenol gave (7) and (8), and 1,3,4,5,6,7,8-heptafluoro-2-naphthol gave (12) and (13).Reaction of polyfluoroarenols with dimethyl sulphoxide--trifluoroacetic anhydride at low temperatures followed by deprotonation with triehylamine resulted in more efficient rearrangement reactions; 4-bromo-3,5,6-trifluoropyridin-2-ol gave the ether (14) and the products of rearrangement both to carbon and nitrogen, (15) (after hydrolysis) and (16) respectively; 2,4,5,6-tetrafluoropyridin-3-ol resulted in the overall replacement of the 2-fluorine by CHO (17) and by CH(SMe)2 (18); and with 2,5,6-trifluoropyrimidin-4-ol and 5-fluoro-4,6-dimethoxypyrimidine-2-ol, simple rearrangement products (21) and (22) were obtained.Hydrolysis of (21) gave the 5-fluorouracil derivative (23).Sodium borohydride reduction and Raney nickel desulphurisations of some of the rearrangement compounds gave phenolic products.Reaction of the sulfone from (13) with base (DBU) effected of the overall efficient replacement of the 1-fluorine in the 2-naphthol by CHO (35) and by CH(SO2Me)2 (36).
