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ALA-ALA-PHE-MCA HYDROCHLORIDE, also known as Ala-Ala-Phe-7-amido-4-methylcoumarin, is a synthetic peptide compound that serves as a substrate for enzymatic assays. It is composed of three amino acids (alanine, alanine, and phenylalanine) and a 7-amido-4-methylcoumarin group, which allows for the detection and measurement of enzyme activity through fluorescence.
Used in Enzymatic Assays:
ALA-ALA-PHE-MCA HYDROCHLORIDE is used as a substrate in enzymatic assays for the detection and measurement of enzyme activity. The fluorescence emitted by the released 7-amido-4-methylcoumarin upon enzymatic cleavage allows for the quantification of enzyme activity in various biological samples.
Used in Tripeptidyl Peptidase-1 (TPP1) Enzyme Activity Assay:
In the TPP1 enzyme activity assay, ALA-ALA-PHE-MCA HYDROCHLORIDE is used as a specific substrate to initiate the enzyme reaction. The TPP1 enzyme cleaves the peptide bond between the phenylalanine and the 7-amido-4-methylcoumarin group, resulting in the release of the fluorescent product. This allows for the assessment of TPP1 enzyme activity and its potential role in various biological processes and diseases.
Used in Lysosomal Hydrolases Activity Assay:
ALA-ALA-PHE-MCA HYDROCHLORIDE is also used in lysosomal hydrolases activity assays as a substrate to initiate the assay reaction. Lysosomal hydrolases are enzymes responsible for the degradation of various biomolecules within lysosomes. The fluorescence emitted by the released 7-amido-4-methylcoumarin upon enzymatic cleavage by lysosomal hydrolases provides a means to evaluate the activity of these enzymes, which is crucial for understanding their role in lysosomal storage disorders and other related diseases.

62037-41-6

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62037-41-6 Usage

Check Digit Verification of cas no

The CAS Registry Mumber 62037-41-6 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 6,2,0,3 and 7 respectively; the second part has 2 digits, 4 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 62037-41:
(7*6)+(6*2)+(5*0)+(4*3)+(3*7)+(2*4)+(1*1)=96
96 % 10 = 6
So 62037-41-6 is a valid CAS Registry Number.
InChI:InChI=1/C25H28N4O5/c1-14-11-22(30)34-21-13-18(9-10-19(14)21)28-25(33)20(12-17-7-5-4-6-8-17)29-24(32)16(3)27-23(31)15(2)26/h4-11,13,15-16,20H,12,26H2,1-3H3,(H,27,31)(H,28,33)(H,29,32)

62037-41-6SDS

SAFETY DATA SHEETS

According to Globally Harmonized System of Classification and Labelling of Chemicals (GHS) - Sixth revised edition

Version: 1.0

Creation Date: Aug 18, 2017

Revision Date: Aug 18, 2017

1.Identification

1.1 GHS Product identifier

Product name Ala-Ala-Phe-7-amido-4-methylcoumarin

1.2 Other means of identification

Product number -
Other names 2-[2-(2-aminopropanoylamino)propanoylamino]-N-(4-methyl-2-oxochromen-7-yl)-3-phenylpropanamide

1.3 Recommended use of the chemical and restrictions on use

Identified uses For industry use only.
Uses advised against no data available

1.4 Supplier's details

1.5 Emergency phone number

Emergency phone number -
Service hours Monday to Friday, 9am-5pm (Standard time zone: UTC/GMT +8 hours).

More Details:62037-41-6 SDS

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