6226-79-5 Usage
Uses
Used in Molecular Biology and Biochemistry:
Ponceau S is used as a reversible protein stain for the rapid and temporary visualization of protein bands on nitrocellulose or PVDF membranes during Western blot analysis. Its reversible nature allows for easy destaining, making it a convenient choice for researchers.
Used in Electrophoresis:
Ponceau S serves as a staining agent for proteins in various electrophoretic techniques, such as SDS-PAGE. It provides a quick and efficient method for visualizing protein bands, aiding in the assessment of protein separation and molecular weight estimation.
Used in Mass Spectrometric Imaging:
Ponceau S is utilized as a fiducial marker in mass spectrometric imaging, a technique used to analyze the spatial distribution of molecules within a sample. Its distinct color and staining properties make it an ideal reference point for calibration and alignment purposes in this analytical method.
Preparation
3,4′-Disulfo-4-aininoazobenzene diazo, coupled with 3-Hydroxynaphthalene-2,7-disulfonic acid.
Standard
Light Fastness
Fading
Stain
ISO
3-4
Check Digit Verification of cas no
The CAS Registry Mumber 6226-79-5 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 6,2,2 and 6 respectively; the second part has 2 digits, 7 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 6226-79:
(6*6)+(5*2)+(4*2)+(3*6)+(2*7)+(1*9)=95
95 % 10 = 5
So 6226-79-5 is a valid CAS Registry Number.
InChI:InChI=1/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;
