635679-17-3

Upstream product

• 17754-90-4 4-(Diethylamino)salicylaldehyde 
• 18162-48-6 tert-butyldimethylsilyl chloride 

Downstream Products

• 1401448-31-4 C₂₆H₃₃N₃OSSi 

Relevant academic research and scientific papers

A selective, sensitive, colorimetric, and fluorescence probe for relay recognition of fluoride and Cu(II) ions with off-On-Off switching in ethanol-water solution

Anion to cation relay recognition was designed and realized for the first time with sequence specificity (F-→Cu2+) via a fluorescence off-on-off mechanism. Probe 1 was a highly selective, sensitive, and turn-on chemodosimeter for F- through a specific cyclization reaction triggered by the strong affinity of fluoride toward silicon with a significant change of fluorescence color in both ethanol and ethanol-water (1:1, v/v) solution. Fluorescence enhancement factors were dramatic: 833-fold in ethanol and 164-fold in ethanol-water (1:1, v/v) solution, respectively. The in situ system generated from the sensing of F- showed good relay recognition ability for Cu2+ via fast fluorescence quenching by the formation of a 1:1 complex in ethanol-water (1:1, v/v) solution. The isolated pure compound 2 also exhibited high selectivity toward Cu2+ in PBS buffer (pH = 7.0) solution. The origin of this sequence specificity of fluorescence recognition was disclosed through the crystal or optimized structures and DFT calculations of corresponding compounds.

Molecular probe for fluorescence quenching detection of fluorine ions and preparation method thereof

The invention discloses a molecular probe for fluorescence quenching detection of fluorine ions and a preparation method thereof. The fluorescent molecular probe is prepared by taking 4-(diethylamino)-2-hydroxy benzaldehyde as a raw material, introducing tert-butyl dimethyl chlorosilane and then condensing with malononitrile. Synthesis is simple, and reaction conditions are mild. The probe is stable in molecular optical performance and high in synthesis yield; the detection sensitivity to fluorine ions is high, the detection lower limit is low, the response range is wide, the detection limit is extremely low, the detection range is wide, the selectivity is good, and no response is made to common cations and interfering anions, and the probe is suitable for naked eye detection. The fluorescent molecular probe has practical application value in the fields of biochemistry, environmental science and the like.

Cyclization Reaction-Based Turn-on Probe for Covalent Labeling of Target Proteins

Fluorescent molecules have contributed to basic biological research but there are currently only a limited number of probes available for the detection of non-enzymatic proteins. Here, we report turn-on fluorescent probes mediated by conjugate addition and cyclization (TCC probes). These probes react with multiple amino acids and exhibit a 36-fold greater emission intensity after reaction. We analyzed the reactions between TCC probes and nuclear receptors by electrospray ionization mass spectrometry, X-ray crystallography, spectrofluorometry, and fluorescence microscopy. In vitro analysis showed that probes consisting of a protein ligand and TCC could label vitamin D receptor and peroxisome proliferator-activated receptor γ. Moreover, we demonstrated that not only a ligand unit but also a peptide unit can label the target protein in a complex mixture. Non-enzymatic proteins are challenging targets for turn-on probes. Here, Kojima et al. report turn-on fluorescent probes mediated by conjugate addition and cyclization (TCC probes). These probes react with nuclear receptors and emit bright fluorescence after the reaction. TCC probes are potent tools for molecular imaging and chemical proteomics.