637-01-4 Usage
Description
N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride (TMPD) is a redox mediator that exists as an off-white to gray powder. It is an oxidizable compound known for its role as a reducing co-substrate for heme peroxidases. TMPD is widely recognized for its applications in various fields, including microbiology, biochemistry, and molecular biology.
Uses
Used in Biochemistry:
TMPD is used as a reducing co-substrate for heme peroxidases, which are enzymes that catalyze the oxidation of various substrates using hydrogen peroxide as an electron acceptor. This application is crucial in studying the mechanisms and functions of these enzymes in biological systems.
Used in Microbiology:
1. In the Classification of Aerobic Microorganisms:
TMPD is used as a test reagent for the classification of cytochrome oxidase positive aerobic microorganisms. This helps in distinguishing between different types of bacteria based on their ability to utilize oxygen in their metabolic processes.
2. In the Detection of Peroxidases on Polyacrylamide Gels:
TMPD is utilized for detecting peroxidases, a group of enzymes that catalyze the oxidation of various organic compounds, on polyacrylamide gels. This application is essential in research and diagnostic laboratories for identifying and characterizing peroxidase enzymes.
3. In Determining Oxidase Activity in Microorganisms:
TMPD has been employed to determine the oxidase activity displayed by various microorganisms. This information is valuable for understanding the metabolic capabilities of these organisms and their potential roles in various ecological and industrial processes.
Purification Methods
Crystallise the salt from isopropyl or n-butyl alcohols, saturated with HCl. Treat it with aqueous NaOH to give the free base (see previous entry) which is filtered, dried and sublimed in a vacuum. [Guarr et al. J Am Chem Soc 107 5104 1985, Beilstein 13 H 74.]
Check Digit Verification of cas no
The CAS Registry Mumber 637-01-4 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 6,3 and 7 respectively; the second part has 2 digits, 0 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 637-01:
(5*6)+(4*3)+(3*7)+(2*0)+(1*1)=64
64 % 10 = 4
So 637-01-4 is a valid CAS Registry Number.
637-01-4Relevant articles and documents
Contact Ion Pairs Formed from Photolyzed TMPD-CCl4 and TMPD-CCl4-C6H6 Solutions Studied by the Time-Resolved Microwave Dielectric Absorption Technique
Shimamori, Hiroshi,Uegaito, Hisakazu
, p. 6218 - 6227 (2007/10/02)
One-photon ionization of TMPD has been investigated by observing the time variation of microwave dielectric loss caused by irradiation of 355-nm laser pulse on a solution of TMPD in CCl4 and on that containing both TMPD and CCl4 in C6H6 solvent.In the TMPD-CCl4 system the observed signal shows a rapid growth followed by a second-order decay along with much slower decays.These features can be interpreted as reflecting the formation of contact ion pairs (TMPD(1+)Cl(1-)) and their mutual association leading to ion-pair dimers and clusters.In the TMPD-CCl4-C6H6 system a first-order growth of the dielectric absorption is observed, which corresponds to ion-pair formation by electron transfer from the excited triplet state of TMPD to CCl4, and the reaction rate is diffusion-controlled.The dipole moment of the ion pair has been estimated to be 11 +/- 3 D from two different methods; one based on the amplitude of the detected signal and the other on the rate constant for the ion-pair association.The dipole moment of the ion pair dimer appears to be larger than that of the ion pair itself.The analysis of the data leads to a suggestion that the quantum yield of the ion-pair formation is close to unity.
