64065-91-4Relevant academic research and scientific papers
Enzymes immobilisees. 14 : Immobilisation de la chymotrypsine prealablement protegee par un inhibiteur macromoleculaire synthetique
Brown, Eric,Loriot, Michel
, p. 481 - 489 (2007/10/02)
A new water-soluble acrylic copolymer 13, with spacer-arms bearing N-(4-phenylbutyl)amido groups, was sythesized and was efficiently used to protect α-chymotrypsin during immobilization of the latter on Acaprosuc, an insoluble cross-linked polyacrylic gel whose side-chains end with reactive N-siccinimidyl ester groups.The insoluble chymotrypsin/Acaprosuc conjugates prepared in the presence of the polyinhibitor 13 were twice as active towards heamoglobin, and 2 to 4 times as active towards ATEE, as the corresponding conjugates prepared in the absence of the polyinhibitor 13.These results represent a generalization of the enzyme protection/immobilization/deprotection method which was developed in our laboratory some years ago in the particular case of trypsin.
