6409-77-4 Usage
Chemical Properties
crystalline powder
Uses
Nuclear Fast Red is used as a biological stain and is suitable for the determination of calcium. Nuclear Fast Red targets nucleic acids and works more quickly than Hematoxylin. Dyes and metabolites.
Definition
ChEBI: An organic sodium salt that is the monosodium salt of 4-amino-1,3-dihydroxy-9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid. It forms bright red lakes with calcium and is a useful red nuclear staining lake with aluminum.
Purification Methods
A solution of 5g of the dye in 250mL of warm 50% EtOH is cooled to 15o for 36hours, then filtered on a Büchner funnel, washed with EtOH until the washings are colourless, then with 100mL of Et2O and dry it over P2O5 in vacuo. It is a biological stain which is also used for the estimation of Ca. [Kingsley & Robnett Anal Chem 33 552 1961.] Octadecyl isonicotinate See hydrogen ionophore IV, ETH 1778 in “Heterocyclic Compounds”, Chapter 4.
Check Digit Verification of cas no
The CAS Registry Mumber 6409-77-4 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 6,4,0 and 9 respectively; the second part has 2 digits, 7 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 6409-77:
(6*6)+(5*4)+(4*0)+(3*9)+(2*7)+(1*7)=104
104 % 10 = 4
So 6409-77-4 is a valid CAS Registry Number.
InChI:InChI=1/C14H9NO7S/c15-9-7-8(12(18)14(13(9)19)23(20,21)22)11(17)6-4-2-1-3-5(6)10(7)16/h1-4,18-19H,15H2,(H,20,21,22)/p-1
