64350-25-0

Upstream product

• 552-89-6 2-nitro-benzaldehyde 
• 64350-24-9 N²-isobutyryl-2'-deoxyguanosine 
• 3958-60-9 2-nitrophenylmethyl bromide 
• 58809-90-8 4-methyl-N'-(2-nitrobenzylidene)-benzenesulfonohydrazide 

Downstream Products

• 1300713-90-9 5'-O-(dimethoxytrityl)-N²-isobutyryl-2'-O-(o-nitrobenzyl)guanosine 
• 1586816-15-0 5'-O-(dimethoxytrityl)-N²-isobutyryl-2'-O-(o-nitrobenzyl)-guanosine 3′-N,N-diisopropyl(cyanoethyl)phosphoramidite 
• 1586816-16-1 5'-O-(tert-butyldiphenylsilyl)-N²-isobutyryl-2'-O-(o-nitrobenzyl)-3'-O-trifluoromethylsulfonylguanosine 
• 1586816-14-9 5'-O-(dimethoxytrityl)-N²-isobutyryl-2'-O-(o-nitrobenzyl)-3'-thioguanosine-3'-S-(cyanoethyl-N,N-diisopropylphosphoramidite) 
• 1586816-13-8 5'-O-(dimethoxytrityl)-N²-isobutyryl-2'-O-(o-nitrobenzyl)-3'-thioguanosine 
• 1586816-12-7 3'-S-acetyl-5'-O-(dimethoxytrityl)-N²-isobutyryl-2′-O-(onitrobenzyl)-3′-thioguanosine 
• 1586816-11-6 3'-S-acetyl-N²-isobutyryl-2'-O-(o-nitrobenzyl)-3'-thioguanosine 
• 1586816-10-5 3'-β-bromo-5'-O-(tert-butyldiphenylsilyl)-N²-isobutyryl-2'-O-(o-nitrobenzyl)guanosine 
• 1586816-09-2 5'-O-(tert-butyldiphenylsilyl)-N²-isobutyryl-2'-O-(o-nitrobenzyl)-3'-O-trifluoromethylsulfonylguanosine 

Relevant academic research and scientific papers

Synthesis of 2′-O-Photocaged Ribonucleoside Phosphoramidites

The chemical synthesis and incorporation of the phosphoramidite derivatives of 2′-O-photocaged ribonucleosides (A, C, G and U) with o-nitrobenzyl, α-methyl-o-nitrobenzyl or 4,5-dimethoxy-2-nitrobenzyl group into oligoribonucleotides are described. The eff

Synthesis and incorporation of the phosphoramidite derivative of 2′- O-photocaged 3′-S-thioguanosine into oligoribonucleotides: Substrate for probing the mechanism of RNA catalysis

Oligoribonucleotides containing 3′-S-phosphorothiolate linkages possess properties that can reveal deep mechanistic insights into ribozyme-catalyzed reactions. Photocaged 3′-S- RNAs could provide a strategy to stall reactions at the chemical stage and release them after assembly steps have occurred. Toward this end, we describe here an approach for the synthesis of 2′-O-(o-nitrobenzyl)-3′-thioguanosine phosphoramidite starting from N2-isobutyrylguanosine in nine steps with 10.2% overall yield. Oligonucleotides containing the 2′-O-(o- nitrobenzyl)-3′-S-guanosine nucleotide were then constructed, characterized, and used in a nuclear pre-mRNA splicing reaction.