64378-95-6Relevant academic research and scientific papers
Development of immunoassays for detecting oxyfluorfen residue in agricultural and environmental samples?
Sheng, Enze,Du, Mei,Yang, Jiachuan,Hua, Xiude,Wang, Minghua
, p. 5020 - 5025 (2018/02/09)
An enzyme-linked immunosorbent assay (ELISA) and chemiluminescent immunoassays (CLEIA) were developed to detect oxyfluorfen in agricultural and environmental samples. The hapten of oxyfluorfen was synthesized and conjugated with bovine serum albumin (BSA) and ovalbumin (OVA) to produce immunogen and coating antigen. One cell line (1A7D6F5) that stably secretes anti-oxyfluorfen monoclonal antibody (mAb) is obtained by cell fusion. Under optimal conditions, the half-maximal inhibition concentration (IC50) and the limit of detection (LOD, IC20) of ELISA are 0.065 mg L1 and 0.0048 mg L1, while those of CLEIA are 0.021 mg L1 and 0.0016 mg L1, respectively. The immunoassays show no obvious cross-reactivities with the analogues of oxyfluorfen except for benzofluorfen and bifenox. The recoveries of oxyfluorfen in the spiked samples of soil, grape, peach, apple and pear are in the range of 74.1–107.2% with a RSD of 2.7–9.7% for ELISA, and 77.2–106.4% with a RSD of 2.4–7.9% for CLEIA. The results of immunoassays for the authentic samples are significantly correlated with those detected by gas chromatography (GC).
Metabolism of nitrodiphenyl ether herbicides by dioxin-degrading bacterium Sphingomonas wittichii RW1
Young, Soo Keum,Young, Ju Lee,Kim, Jeong-Han
experimental part, p. 9146 - 9151 (2010/04/23)
Nitrodiphenyl ether herbicides, including chlomethoxyfen, nitrofen, and oxyfluorfen are potent herbicides. Some metabolites and parent compounds are considered as possible mutagens and endocrine disruptors. Both properties pose serious hygienic and environmental risks. Sphingomonas wittichii RW1 is a well-known degrader of polychlorinated dibenzo-p-dioxins, dibenzofurans, and diphenyl ethers. However, no detailed research of its metabolic activity has been performed against pesticides with a diphenyl ether scaffold. In this study, we report S. wittichii RW1 as a very potent diphenyl ether herbicide- metabolizing bacterium with broad substrate specificity. The structures of metabolites were determined by instrumental analysis and synthetic standards. Most pesticides were rapidly removed from the culture medium in the order of nitrofen > oxyfluorfen > chlomethoxyfen. In general, herbicides were degraded through the initial reduction and N-acetylation of nitro groups, followed by ether bond cleavage. Relatively low concentrations of phenolic and catecholic metabolites throughout the study suggested that these metabolites were rapidly metabolized and incorporated into primary metabolism. These results indicate that strain RW1 has very versatile metabolic activities over a wide range of environmental contaminants.
